Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab176694 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 59 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionRegulates, cooperatively with NONO and SFPQ, androgen receptor-mediated gene transcription activity in Sertoli cell line (By similarity). Binds to poly(A), poly(G) and poly(U) RNA homopolymers.
Tissue specificityExpressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart.
Sequence similaritiesBelongs to the PSPC family.
Contains 2 RRM (RNA recognition motif) domains.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus > nucleolus. Nucleus matrix. Cytoplasm. In punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles. Colocalizes with NONO and SFPQ in paraspeckles and perinucleolar caps in a RNA-dependent manner. May cycles between paraspeckles and nucleolus. In telophase, when daughter nuclei form, localizes to perinucleolar caps.
- Information by UniProt
- Paraspeckle component 1 antibody
- Paraspeckle protein 1 antibody
- PSP1 antibody
Detection of Human PSPC1 by Western Blot of Immunoprecipitates. 1 mg for IP; 20% of IP HeLa whole cell lysate loaded. ab176694 used for IP at 6 µg/mg lysate. For blotting immunoprecipitated PSPC1, ab176694 was used at 0.4 µg/ml.
All lanes : Anti-PSPC1 antibody (ab176694) at 1/0.04 dilution
Lane 1 : HeLa lysate at 50 µg
Lane 2 : HeLa lysate at 15 µg
Lane 3 : 293T lysate at 50 µg
Lane 4 : Jurkat lysate at 50 µg
Lane 5 : Mouse NIH3T3 lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 59 kDa
Exposure time: 3 minutes
ab176694 has not yet been referenced specifically in any publications.