Regulates, cooperatively with NONO and SFPQ, androgen receptor-mediated gene transcription activity in Sertoli cell line (By similarity). Binds to poly(A), poly(G) and poly(U) RNA homopolymers.
Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart.
Belongs to the PSPC family. Contains 2 RRM (RNA recognition motif) domains.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Nucleus > nucleolus. Nucleus matrix. Cytoplasm. In punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles. Colocalizes with NONO and SFPQ in paraspeckles and perinucleolar caps in a RNA-dependent manner. May cycles between paraspeckles and nucleolus. In telophase, when daughter nuclei form, localizes to perinucleolar caps.
Detection of Human PSPC1 by Western Blot of Immunoprecipitates. 1 mg for IP; 20% of IP HeLa whole cell lysate loaded. ab176694 used for IP at 6 µg/mg lysate. For blotting immunoprecipitated PSPC1, ab176694 was used at 0.4 µg/ml.
Western blot - Anti-PSPC1 antibody (ab176694)
All lanes : Anti-PSPC1 antibody (ab176694) at 1/0.04 dilution
Lane 1 : HeLa lysate at 50 µg Lane 2 : HeLa lysate at 15 µg Lane 3 : 293T lysate at 50 µg Lane 4 : Jurkat lysate at 50 µg Lane 5 : Mouse NIH3T3 lysate at 50 µg