Overview

  • Product name

  • Description

    Rabbit polyclonal to PSPH
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Recombinant fragment within Human PSPH aa 1-175. The exact sequence is proprietary. NCBI Accession No. NP_004568.2.
    Sequence:

    MVSHSELRKL FYSADAVCFD VDSTVIREEG IDELAKICGV EDAVSEMTRR AMGGAVPFKA ALTERLALIQ PSREQVQRLI AEQPPHLTPG IRELVSRLQE RNVQVFLISG GFRSIVEHVA SKLNIPATNV FANRLKFYFN GEYAGFDETQ PTAESGGKGK VIKLLKEKFH FKKII


    Database link: P78330

  • Positive control

    • A431 whole cell lysate, Human kidney tissue and HeLa cells.
  • General notes

    Previously labelled as Phosphoserine phosphatase.

Properties

Applications

Our Abpromise guarantee covers the use of ab211418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 7.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500 - 1/3000. Predicted molecular weight: 25 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Catalyzes the last step in the biosynthesis of serine from carbohydrates. The reaction mechanism proceeds via the formation of a phosphoryl-enzyme intermediates.
  • Pathway

    Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 3/3.
  • Involvement in disease

    Defects in PSPH are the cause of phosphoserine phosphatase deficiency (PSPHD)[MIM:614023]. A disorder that results in pre- and postnatal growth retardation, moderate psychomotor retardation and facial features suggestive of Williams syndrome.
  • Sequence similarities

    Belongs to the SerB family.
  • Information by UniProt
  • Database links

  • Alternative names

    • EC 3.1.3.3 antibody
    • L 3 phosphoserine phosphatase antibody
    • L-3-phosphoserine phosphatase antibody
    • O phosphoserine phosphohydrolase antibody
    • O-phosphoserine phosphohydrolase antibody
    • Phosphoserine phosphatase antibody
    • Phosphoserine phosphatase deficiency, included antibody
    • PSP antibody
    • PSPase antibody
    • Psph antibody
    • PSPHD antibody
    • SERB_HUMAN antibody
    see all

Images

  • Anti-PSPH antibody (ab211418) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 25 kDa



    12% SDS PAGE

  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling PSPH using ab211418 at a 1/200 dilution (green, above). The image below was costained with a DNA probe (blue).

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling PSPH using ab211418 at 7.5 µg/ml.

References

ab211418 has not yet been referenced specifically in any publications.

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