Key features and details
- Rabbit polyclonal to PSPH
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PSPH antibody
See all PSPH primary antibodies
DescriptionRabbit polyclonal to PSPH
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
Recombinant fragment within Human PSPH aa 1-175. The exact sequence is proprietary. NCBI Accession No. NP_004568.2.
MVSHSELRKL FYSADAVCFD VDSTVIREEG IDELAKICGV EDAVSEMTRR AMGGAVPFKA ALTERLALIQ PSREQVQRLI AEQPPHLTPG IRELVSRLQE RNVQVFLISG GFRSIVEHVA SKLNIPATNV FANRLKFYFN GEYAGFDETQ PTAESGGKGK VIKLLKEKFH FKKII
Database link: P78330
- A431 whole cell lysate, Human kidney tissue and HeLa cells.
Previously labelled as Phosphoserine phosphatase.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 1.79% Tris glycine
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab211418 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 7.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/500 - 1/3000. Predicted molecular weight: 25 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionCatalyzes the last step in the biosynthesis of serine from carbohydrates. The reaction mechanism proceeds via the formation of a phosphoryl-enzyme intermediates.
PathwayAmino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 3/3.
Involvement in diseaseDefects in PSPH are the cause of phosphoserine phosphatase deficiency (PSPHD)[MIM:614023]. A disorder that results in pre- and postnatal growth retardation, moderate psychomotor retardation and facial features suggestive of Williams syndrome.
Sequence similaritiesBelongs to the SerB family.
- Information by UniProt
- EC 22.214.171.124 antibody
- L 3 phosphoserine phosphatase antibody
- L-3-phosphoserine phosphatase antibody
Anti-PSPH antibody (ab211418) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 25 kDa
12% SDS PAGE
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling PSPH using ab211418 at a 1/200 dilution (green, above). The image below was costained with a DNA probe (blue).
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling PSPH using ab211418 at 7.5 µg/ml.
ab211418 has not yet been referenced specifically in any publications.