Anti-PSPH antibody (ab224110)
Key features and details
- Rabbit polyclonal to PSPH
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PSPH antibody
See all PSPH primary antibodies -
Description
Rabbit polyclonal to PSPH -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Orangutan -
Immunogen
Recombinant fragment corresponding to Human PSPH aa 1-150.
Database link: P78330 -
Positive control
- WB: A431 cell lysate. IHC-P: Human Fallopian tube, kidney and endometrium tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224110 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 25 kDa.
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 25 kDa. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Catalyzes the last step in the biosynthesis of serine from carbohydrates. The reaction mechanism proceeds via the formation of a phosphoryl-enzyme intermediates. -
Pathway
Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 3/3. -
Involvement in disease
Defects in PSPH are the cause of phosphoserine phosphatase deficiency (PSPHD)[MIM:614023]. A disorder that results in pre- and postnatal growth retardation, moderate psychomotor retardation and facial features suggestive of Williams syndrome. -
Sequence similarities
Belongs to the SerB family. - Information by UniProt
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Database links
- Entrez Gene: 533630 Cow
- Entrez Gene: 5723 Human
- Entrez Gene: 100678 Mouse
- Entrez Gene: 100189699 Orangutan
- Entrez Gene: 304429 Rat
- Omim: 172480 Human
- SwissProt: Q2KHU0 Cow
- SwissProt: P78330 Human
see all -
Alternative names
- EC 3.1.3.3 antibody
- L 3 phosphoserine phosphatase antibody
- L-3-phosphoserine phosphatase antibody
see all
Images
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Paraffin-embedded human Fallopian tube tissue stained for PSPH using ab224110 at 1/500 dilution in immunohistochemical analysis.
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All lanes : Anti-PSPH antibody (ab224110) at 0.04 µg/ml
Lane 1 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Predicted band size: 25 kDaWestern blot analysis labeling PSPH with ab224110 at 0.04 ug/ml.
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Paraffin-embedded human kidney tissue stained for PSPH using ab224110 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded human endometrium tissue stained for PSPH using ab224110 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded human skeletal muscle tissue stained for PSPH using ab224110 at 1/500 dilution in immunohistochemical analysis. No positivity observed.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab224110 has been referenced in 1 publication.
- Huang MY et al. Phosphoserine phosphatase as a prognostic biomarker in patients with gastric cancer and its potential association with immune cells. BMC Gastroenterol 22:1 (2022). PubMed: 34979926