Overview

  • Product name

  • Description

    Rabbit polyclonal to PSRC1/DDA3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Recombinant fragment within Human PSRC1/DDA3 (internal sequence). The exact sequence is proprietary.
    Database link: Q6PGN9

  • Positive control

    • WB: HEK-293T and A431 whole cell extracts; PSRC1/DDA3-transfected HEK-293T whole cell extract. ICC/IF: HeLa cells
  • General notes

     This product was previously labelled as PSRC1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab229742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
WB 1/500 - 1/20000. Predicted molecular weight: 39 kDa.

Target

  • Function

    Required for normal progression through mitosis. Required for normal congress of chromosomes at the metaphase plate, and for normal rate of chromosomal segregation during anaphase. Plays a role in the regulation of mitotic spindle dynamics. Increases the rate of turnover of microtubules on metaphase spindles, and contributes to the generation of normal tension across sister kinetochores. Recruits KIF2A to the mitotic spindle and spindle poles. May participate in p53/TP53-regulated growth suppression.
  • Tissue specificity

    Widely expressed in adult and fetal tissues, with highest expression in the adult brain and fetal thymus. Not detected in adult skeletal muscle.
  • Sequence similarities

    Belongs to the PSRC1 family.
  • Post-translational
    modifications

    Phosphorylated during mitosis.
  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Detected at the mitotic spindle and spindle poles. Diffusely distributed throughout the cell during interphase.
  • Information by UniProt
  • Database links

  • Alternative names

    • DDA3 antibody
    • DDA3, mouse, homolog of antibody
    • Differential display and activated by p53 antibody
    • FP3214 antibody
    • OTTHUMP00000013785 antibody
    • OTTHUMP00000013786 antibody
    • p53-regulated DDA3 antibody
    • Proline/serine-rich coiled-coil 1 antibody
    • Proline/serine-rich coiled-coil protein 1 antibody
    • PSRC1 antibody
    • PSRC1_HUMAN antibody
    see all

Images

  • All lanes : Anti-PSRC1/DDA3 antibody (ab229742) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 39 kDa



    10% SDS-PAGE gel.

  • All lanes : Anti-PSRC1/DDA3 antibody (ab229742) at 1/10000 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : PSRC1/DDA3-transfected HEK-293T whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 39 kDa



    12% SDS-PAGE gel.

  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PSRC1/DDA3 (green) using ab229742 at 1/500 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

References

ab229742 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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