Recombinant Anti-PSRC2 antibody [EPR20913-32] - BSA and Azide free (ab270035)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20913-32] to PSRC2 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PSRC2 antibody [EPR20913-32] - BSA and Azide free
See all PSRC2 primary antibodies -
Description
Rabbit monoclonal [EPR20913-32] to PSRC2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt (Intra),ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse lymph node(WT) and testis tissue lysates; Rat thymus tissue lysate; HeLa; 293T whole cell lysates.
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General notes
ab270035 is the carrier-free version of ab236629.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20913-32 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab270035 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 226 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 226 kDa. |
Target
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Sequence similarities
Contains 1 C3H1-type zinc finger.
Contains 6 HAT repeats.
Contains 5 TPR repeats. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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Database links
- Entrez Gene: 196441 Human
- Entrez Gene: 216345 Mouse
- SwissProt: O60293 Human
- Unigene: 245798 Human
- Unigene: 527874 Human
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Alternative names
- BC033596 antibody
- CCDC 131 antibody
- CCDC131 antibody
see all
Images
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All lanes : Anti-PSRC2 antibody [EPR20913-32] (ab236629) at 1/1000 dilution
Lane 1 : Mouse testis tissue lysate
Lane 2 : Rat thymus tissue lysate
Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 226 kDa
Observed band size: 260 kDa why is the actual band size different from the predicted?This data was developed using ab236629, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 15 seconds
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All lanes : Anti-PSRC2 antibody [EPR20913-32] (ab236629) at 1/1000 dilution
Lane 1 : Mouse lymph node(WT) tissue lysate
Lane 2 : PSRC2 knockout mouse lymph node tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 226 kDa
Observed band size: 260 kDa why is the actual band size different from the predicted?This data was developed using ab236629, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lysates were kindly provided by collaborator.
ab236629 Anti-PSRC2 antibody [EPR20913-32] was shown to react with PSRC2 in mouse lymph node tissue lysate in Western blot. Loss of signal was observed when PSRC2 knockout sample was used. Wild-type and PSRC2 knockout samples were subjected to SDS-PAGE. ab236629 and ab129002 (Rabbit anti-Vinculin loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/5000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100, 000 dilution for 1 hour at room temperature before imaging. he blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
Exposure time: 15 seconds
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab270035 has not yet been referenced specifically in any publications.