Recombinant Anti-PSS-2 antibody [EPR14663] - C-terminal (ab183504)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14663] to PSS-2 - C-terminal
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PSS-2 antibody [EPR14663] - C-terminal
See all PSS-2 primary antibodies -
Description
Rabbit monoclonal [EPR14663] to PSS-2 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2 lysates, Human fetal brain tissue lysate, Human fetal liver tissue lysate. IP: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14663 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 56 kDa).
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IP |
1/30 - 1/70.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 56 kDa). |
IP
1/30 - 1/70. |
Target
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Function
Catalyzes a base-exchange reaction in which the polar head group of phosphatidylethanolamine is replaced by L-serine. -
Pathway
Phospholipid metabolism; phosphatidylserine biosynthesis. -
Sequence similarities
Belongs to the phosphatidyl serine synthase family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 81490 Human
- Omim: 612793 Human
- SwissProt: Q9BVG9 Human
- Unigene: 12851 Human
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Alternative names
- FLJ46373 antibody
- Phosphatidylserine synthase 2 antibody
- PSS 2 antibody
see all
Images
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Anti-PSS-2 antibody [EPR14663] - C-terminal (ab183504) at 1/5000 dilution (Purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted? -
ab183504 (purified ) at 1/30 dilution (2ug) immunoprecipitating PSS-2 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug
Lane 2 (+): ab183504 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab183504 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
Immunoprecipitation analysis of HepG2 cells labeling PSS-2
using ab183504 at 1/50 dilution (lane 1). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.Rabbit monoclonal IgG (ab172730) instead of ab183504 in HepG2 whole cell lysate (Lane 2)
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Anti-PSS-2 antibody [EPR14663] - C-terminal (ab183504) at 1/10000 dilution + Hela cell line lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 56 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted? -
All lanes : Anti-PSS-2 antibody [EPR14663] - C-terminal (ab183504) at 1/2000 dilution
Lane 1 : HepG2 cell line lysate
Lane 2 : Human fetal brain tissue lysate
Lane 3 : Human fetal liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lane 1 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Lanes 2-3 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated
Predicted band size: 56 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab183504 has been referenced in 1 publication.
- Seneviratne AK et al. The Mitochondrial Transacylase, Tafazzin, Regulates for AML Stemness by Modulating Intracellular Levels of Phospholipids. Cell Stem Cell 24:621-636.e16 (2019). PubMed: 30930145