Recombinant
RabMAb

Recombinant Anti-PSS-2 antibody [EPR14663] - C-terminal (ab183504)

Overview

  • Product name

    Anti-PSS-2 antibody [EPR14663] - C-terminal
    See all PSS-2 primary antibodies
  • Description

    Rabbit monoclonal [EPR14663] to PSS-2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human PSS-2 aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q9BVG9

  • Positive control

    • WB: HepG2 lysates, Human fetal brain tissue lysate, Human fetal liver tissue lysate. IP: HeLa cells.
  • General notes

     

     

    Previously labelled as PTDSS2. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14663
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 56 kDa).
IP 1/30 - 1/70.

Target

  • Function

    Catalyzes a base-exchange reaction in which the polar head group of phosphatidylethanolamine is replaced by L-serine.
  • Pathway

    Phospholipid metabolism; phosphatidylserine biosynthesis.
  • Sequence similarities

    Belongs to the phosphatidyl serine synthase family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ46373 antibody
    • Phosphatidylserine synthase 2 antibody
    • PSS 2 antibody
    • PSS-2 antibody
    • PSS2 antibody
    • PtdSer synthase 2 antibody
    • PTDSS 2 antibody
    • PTDSS2 antibody
    • PTSS2_HUMAN antibody
    • Serine-exchange enzyme II antibody
    see all

Images

  • ab183504 (purified ) at 1/30 dilution (2ug) immunoprecipitating PSS-2 in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug
    Lane 2 (+): ab183504 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab183504 in HeLa whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP)�(ab131366) was used at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.
  • Immunoprecipitation analysis of HepG2 cells labeling PSS-2
    using ab183504 at 1/50 dilution (lane 1). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.

    Rabbit monoclonal IgG (ab172730) instead of ab183504 in HepG2 whole cell lysate (Lane 2)

  • Anti-PSS-2 antibody [EPR14663] - C-terminal (ab183504) at 1/10000 dilution + Hela cell line lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 56 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?

  • All lanes : Anti-PSS-2 antibody [EPR14663] - C-terminal (ab183504) at 1/2000 dilution

    Lane 1 : HepG2 cell line lysate
    Lane 2 : Human fetal brain tissue lysate
    Lane 3 : Human fetal liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
    Lanes 2-3 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

    Predicted band size: 56 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?

References

ab183504 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab183504.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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