• Product name

    Anti-PSTAIR antibody [PSTAIR]
  • Description

    Mouse monoclonal [PSTAIR] to PSTAIR
  • Host species

  • Tested applications

    Suitable for: ELISA, IP, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human, Pig, Saccharomyces cerevisiae, Xenopus laevis, Monkey, Plants, Quail
    Predicted to work with: Fish, a wide range of other species
  • Immunogen

    Synthetic peptide:


    conjugated to BSA, corresponding to amino acids 42-57 of Human PSTAIR.

  • Epitope

    Recognizes an epitope present in the PSTAIR sequence 1 of p34cdc2 (cdk1) and of other cyclin-dependent kinases containing the PSTAIR motif (cdk2 and cdk3).



Our Abpromise guarantee covers the use of ab10345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ELISA: Use at an assay dependent dilution.
    ICC/IF: Use at an assay dependent dilution (PMID 19187565).
    IP: Use at an assay dependent dilution. It cannot precipitate p34cdc2 when it is complexed with cyclin B.
    WB: 1/4000 (determined by indirect blotting using COS-7 cell extract). The antibody recognises 31-34kD proteins (1-4) bands.

    Monoclonal Anti-PSTAIR may be used for the localization of cyclin-dependent kinases, containing the PSTAIR motif.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      The cell cycle is controlled in part by cyclin dependent kinases (CDKs) which are themselves controlled by modifications such as phosphorylation of CDKs and formation of complex(es) with other proteins, including the cyclins. CDKs are key regulators of cell cycle progression. CDKs are closely related in size (35-40 kD) and sequence (>40% identity) and associate with and are activated by a cyclin which acts as a regulatory subunit. In every eukaryote examined, CDKs contain an evolutionary conserved 16 amino acid sequence called PSTAIR (EGVPSTAIREISLLKE) which distinguishes them from other protein kinases. The PSTAIR motif is involved in the complex formation with cyclins. The availability of antibodies reacting specifically with the PSTAIR sequence enables the subcellular detection and localization of the various CDKs and examination of substrate interactions, in a variety of organisms.
    • Alternative names

      • EGVPSTAIREISLLKE antibody
      • PSTAIR motif antibody
      • PSTAIR sequence antibody
      • PSTAIRE antibody


    • All lanes : Anti-PSTAIR antibody [PSTAIR] (ab10345) at 1/6000 dilution

      Lane 1 : Whole cell lysate from wild-type S. cerevisiae yeast cells
      Lane 2 : Supernatant after incubation with Protein A-Sepharose beads
      Lane 3 : Eluate from Protein A-Sepharose beads after treatment with TEV protease
      Lane 4 : Product of boiling Protein A-Sepharose after TEV treatment

      All lanes : Goat anti-mouse HRP conjugate

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 31 kDa
      Observed band size: 32,34 kDa
      why is the actual band size different from the predicted?

      Exposure time: 1 minute

      This image is courtesy of an Abreview submitted by Mark Chee on 27 January 2006.

      See Abreview

    • ab10345 staining PSTAIR in Porcine Ooocytes by Immunocytochemistry/ Immunofluorescence. Oocytes were partially denuded by gentle pipettting in PEM-PVPc (80 mM PIPES, 5mM EGTA. 2 mM MgCl2, pH 6.8, supplemented with 0.3% (w/v) PVP) supplemented with either 0.01% (w/v) pronase (for < 22 h cultured oocytes), or 0.1% (w/v) hyaluronidase (for ≥ 22 h cultured oocytes). After washing in PEM-PVP at 37°C, oocytes were fixed in freshly prepared PEM-PVP containing 4% (v/v) paraformaldehyde at room temperature (RT) for 1 h. An Alexa Fluor® 488 conjugated Goat anti mouse IgG was used as secondary. ab10345 show green PSTAIR staining and DNA was stained blue. Arrow indicate the area that is shown enlarged in the inset. Left image show stainming with ab10345 and right one show staining with mouse IgG as negative control.


    This product has been referenced in:

    • Varshney N  et al. Spatio-temporal regulation of nuclear division by Aurora B kinase Ipl1 in Cryptococcus neoformans. PLoS Genet 15:e1007959 (2019). Read more (PubMed: 30763303) »
    • Garcia-Santamarina S  et al. Cryptococcus neoformans Iron-Sulfur Protein Biogenesis Machinery Is a Novel Layer of Protection against Cu Stress. MBio 8:N/A (2017). WB . Read more (PubMed: 29089435) »
    See all 13 Publications for this product

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A


    Thank you for contacting us.

    1. The alternative to Alexa Fluor® 488 goat anti-mouse Ig Cat # G A-11001 is ab96871; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-DyLight-488-ab96871.html

    or ab96879; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-DyLight-488-pre-adsorbed-ab96879.html

    2. ab96895; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-DyLight-488-pre-adsorbed-ab96895.html

    3. ab97035; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-Cy3-pre-adsorbed-ab97035.html

    4. ab97075; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-Cy3-pre-adsorbed-ab97075.html

    5. ab10345; https://www.abcam.com/PSTAIR-antibody-PSTAIR-ab10345.html

    6. We do not have anti Nanog mouse monoclonal that customer is after. We have similar rabbit polyclonal antibodies available. The links are



    7. ab109187; https://www.abcam.com/Oct4-antibody-EPR2053-2-ab109187.html

    8. ab97959; https://www.abcam.com/SOX2-antibody-ab97959.html

    9. ab131000; https://www.abcam.com/Caspase-3-antibody-ab131000.html

    10. ab18737; https://www.abcam.com/Caspase-2-antibody-ab18737.html

    11. We do not sell this chemical.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!

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    Thank you very much for contacting us.

    We recommend making small aliquots e.g. 10ul or 5 ul each when storing the antibody. For extra stability glycerol at 1:1 ration can be added.

    I hope this information will be helpful. Should you have any question please do not hesitate to contact me.

    Read More


    Thank you for your enquiry. I am sorry but we do not have a western image of ab20994. However, I have received an image of ab24702 applied using wild type yeast strains and a temperature sensitive strain. I have uploaded it to the antibody datasheet and it is viewable at the weblink below. Have you considered the use of our immunogen affinity purified antibody; Histone H3 antibody - ChIP Grade (ab1791) as a loading control? This has been successfully applied to yeast lysates. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More
    Western blot
    Saccharomyces cerevisiae Cell lysate - whole cell
    Blocking step
    Milk as blocking agent for 45 minute(s) · Concentration: 5%

    Mr. Mark Chee

    Verified customer

    Submitted Jan 27 2006


    Thank you for your enquiry. This antibody cross-reacts with Human, Mouse, Rat, Xenopus laevis, Cow, Fish, Monkey, Plants, Quail and a wide range of other species. It thus should detect PSTAIR in Arabidopsis. However, I do not know if PSTAIR is ubiquitously expressed in Arabidopsis and would suggest that you refer to the latest literature in the field. If you need further assistance, please let us know.

    Read More

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