Product nameAnti-PSTAIR antibody [PSTAIR]
DescriptionMouse monoclonal [PSTAIR] to PSTAIR
Tested applicationsSuitable for: ELISA, IP, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Cow, Human, Pig, Saccharomyces cerevisiae, Xenopus laevis, Monkey, Plants, Quail
Predicted to work with: Fish, a wide range of other species
EpitopeRecognizes an epitope present in the PSTAIR sequence 1 of p34cdc2 (cdk1) and of other cyclin-dependent kinases containing the PSTAIR motif (cdk2 and cdk3).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.097% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab10345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution (PMID 19187565).
IP: Use at an assay dependent dilution. It cannot precipitate p34cdc2 when it is complexed with cyclin B.
WB: 1/4000 (determined by indirect blotting using COS-7 cell extract). The antibody recognises 31-34kD proteins (1-4) bands.
Monoclonal Anti-PSTAIR may be used for the localization of cyclin-dependent kinases, containing the PSTAIR motif.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceThe cell cycle is controlled in part by cyclin dependent kinases (CDKs) which are themselves controlled by modifications such as phosphorylation of CDKs and formation of complex(es) with other proteins, including the cyclins. CDKs are key regulators of cell cycle progression. CDKs are closely related in size (35-40 kD) and sequence (>40% identity) and associate with and are activated by a cyclin which acts as a regulatory subunit. In every eukaryote examined, CDKs contain an evolutionary conserved 16 amino acid sequence called PSTAIR (EGVPSTAIREISLLKE) which distinguishes them from other protein kinases. The PSTAIR motif is involved in the complex formation with cyclins. The availability of antibodies reacting specifically with the PSTAIR sequence enables the subcellular detection and localization of the various CDKs and examination of substrate interactions, in a variety of organisms.
- EGVPSTAIREISLLKE antibody
- PSTAIR motif antibody
- PSTAIR sequence antibody
- PSTAIRE antibody
All lanes : Anti-PSTAIR antibody [PSTAIR] (ab10345) at 1/6000 dilution
Lane 1 : Whole cell lysate from wild-type S. cerevisiae yeast cells
Lane 2 : Supernatant after incubation with Protein A-Sepharose beads
Lane 3 : Eluate from Protein A-Sepharose beads after treatment with TEV protease
Lane 4 : Product of boiling Protein A-Sepharose after TEV treatment
All lanes : Goat anti-mouse HRP conjugate
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 32,34 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
This image is courtesy of an Abreview submitted by Mark Chee on 27 January 2006.
ab10345 staining PSTAIR in Porcine Ooocytes by Immunocytochemistry/ Immunofluorescence. Oocytes were partially denuded by gentle pipettting in PEM-PVPc (80 mM PIPES, 5mM EGTA. 2 mM MgCl2, pH 6.8, supplemented with 0.3% (w/v) PVP) supplemented with either 0.01% (w/v) pronase (for < 22 h cultured oocytes), or 0.1% (w/v) hyaluronidase (for ≥ 22 h cultured oocytes). After washing in PEM-PVP at 37°C, oocytes were fixed in freshly prepared PEM-PVP containing 4% (v/v) paraformaldehyde at room temperature (RT) for 1 h. An Alexa Fluor® 488 conjugated Goat anti mouse IgG was used as secondary. ab10345 show green PSTAIR staining and DNA was stained blue. Arrow indicate the area that is shown enlarged in the inset. Left image show stainming with ab10345 and right one show staining with mouse IgG as negative control.
This product has been referenced in:
- Varshney N et al. Spatio-temporal regulation of nuclear division by Aurora B kinase Ipl1 in Cryptococcus neoformans. PLoS Genet 15:e1007959 (2019). Read more (PubMed: 30763303) »
- Garcia-Santamarina S et al. Cryptococcus neoformans Iron-Sulfur Protein Biogenesis Machinery Is a Novel Layer of Protection against Cu Stress. MBio 8:N/A (2017). WB . Read more (PubMed: 29089435) »