Anti-PSTPIP2 antibody (ab155543)

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Western blot - Anti-PSTPIP2 antibody (ab155543)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSTPIP2 antibody (ab155543)

Key features and details

  • Rabbit polyclonal to PSTPIP2
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-PSTPIP2 antibody
    See all PSTPIP2 primary antibodies

  • Description

    Rabbit polyclonal to PSTPIP2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-334 of Human PSTPIP2 (Uniprot ID: Q9H939)

  • Positive control

    • Human colon cancer tissue; Raw264.7 whole cell lysate.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 39 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.

Target

  • Relevance

    PSTPIP2 (Proline-serine-threonine phosphatase-interacting protein 2) binds to F-actin and may be involved in regulation of the actin cytoskeleton.

  • Cellular localization

    Cell Membrane; peripheral membrane protein and Cytoplasmic (by similarity).

  • Database links

    • Alternative names

      • Macrophage actin associated tyrosine phosphorylated protein antibody
      • Macrophage actin-associated tyrosine-phosphorylated protein antibody
      • MAYP antibody
      • Proline serine threonine phosphatase interacting protein 2 antibody
      • Proline-serine-threonine phosphatase-interacting protein 2 antibody
      see all

    Images

    • Western blot - Anti-PSTPIP2 antibody (ab155543)
      Western blot - Anti-PSTPIP2 antibody (ab155543)
      All lanes : Anti-PSTPIP2 antibody (ab155543) at 1/2000 dilution

      Lane 1 : Raw264.7 whole cell lysate

      Lane 2 : Raw264.7 knock down PSTPIP2 whole cell lysate


      Lysates/proteins at 20 µg per lane.

      Predicted band size: 39 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSTPIP2 antibody (ab155543)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSTPIP2 antibody (ab155543)
      Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue, labeling PSTPIP2 with ab155543 at 1/250 dilution.

    References (2)

    Publishing research using ab155543? Please let us know so that we can cite the reference in this datasheet.

    ab155543 has been referenced in 2 publications.

    • Yao Y  et al. PSTPIP2 attenuates joint damage and suppresses inflammation in adjuvant-induced arthritis. Eur J Pharmacol 859:172558 (2019). PubMed: 31325437
    • Yang Y  et al. PSTPIP2 connects DNA methylation to macrophage polarization in CCL4-induced mouse model of hepatic fibrosis. Oncogene N/A:N/A (2018). PubMed: 29993036

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