Anti-PSTPIP2 antibody (ab155543)

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Overview

  • Product name

    Anti-PSTPIP2 antibody
    See all PSTPIP2 primary antibodies

  • Description

    Rabbit polyclonal to PSTPIP2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-334 of Human PSTPIP2 (Uniprot ID: Q9H939)

  • Positive control

    • Human colon cancer tissue; Raw264.7 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab155543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 39 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.

Target

  • Relevance

    PSTPIP2 (Proline-serine-threonine phosphatase-interacting protein 2) binds to F-actin and may be involved in regulation of the actin cytoskeleton.

  • Cellular localization

    Cell Membrane; peripheral membrane protein and Cytoplasmic (by similarity).

  • Database links

    • Alternative names

      • Macrophage actin associated tyrosine phosphorylated protein antibody
      • Macrophage actin-associated tyrosine-phosphorylated protein antibody
      • MAYP antibody
      • Proline serine threonine phosphatase interacting protein 2 antibody
      • Proline-serine-threonine phosphatase-interacting protein 2 antibody
      see all

    Images

    • Western blot - Anti-PSTPIP2 antibody (ab155543)
      Western blot - Anti-PSTPIP2 antibody (ab155543)
      All lanes : Anti-PSTPIP2 antibody (ab155543) at 1/2000 dilution

      Lane 1 : Raw264.7 whole cell lysate

      Lane 2 : Raw264.7 knock down PSTPIP2 whole cell lysate


      Lysates/proteins at 20 µg per lane.

      Predicted band size: 39 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSTPIP2 antibody (ab155543)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSTPIP2 antibody (ab155543)
      Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue, labeling PSTPIP2 with ab155543 at 1/250 dilution.

    References

    This product has been referenced in:

    • Yao Y  et al. PSTPIP2 attenuates joint damage and suppresses inflammation in adjuvant-induced arthritis. Eur J Pharmacol 859:172558 (2019). Read more (PubMed: 31325437) »
    • Yang Y  et al. PSTPIP2 connects DNA methylation to macrophage polarization in CCL4-induced mouse model of hepatic fibrosis. Oncogene N/A:N/A (2018). Read more (PubMed: 29993036) »
    See all 2 Publications for this product

    Publishing research using ab155543? Please let us know so that we can cite the reference in this datasheet.

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