Key features and details
- Rabbit polyclonal to PTBP1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PTBP1 antibody
See all PTBP1 primary antibodies
DescriptionRabbit polyclonal to PTBP1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Pig, Xenopus tropicalis
Recombinant fragment corresponding to a region within amino acids 293-528 of Human PTBP1 (UniProt ID: P26599).
- 293T, A431, HeLa and HepG2 whole cell lysate. Human AGS xenograft tissue.
General notesKeep as concentrated solution.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154887 in the following tested applications.
|WB||1/500 - 1/3000. Predicted molecular weight: 57 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPlays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA.
Sequence similaritiesContains 4 RRM (RNA recognition motif) domains.
- Information by UniProt
- 57 kDa RNA binding protein PPTB 1 antibody
- 57 kDa RNA-binding protein PPTB-1 antibody
- Heterogeneous nuclear ribonucleoprotein I antibody
All lanes : Anti-PTBP1 antibody (ab154887) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 57 kDa
7.5% SDS PAGE
Immunohistochemical analysis of paraffin embedded Human AGS xenograft tissue, labeling PTBP1 using ab154887 at 1/500 dilution.
ab154887 has not yet been referenced specifically in any publications.