Overview

  • Product name

    Anti-PTBP1 antibody [EPR9048(B)]
    See all PTBP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR9048(B)] to PTBP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ChIP, WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to a region within Human PTBP1 (UniProt ID: P26599).

  • Positive control

    • Human fetal liver, Jurkat cell, Daudi cell, A549 and BxPC3 cell lysates; Human breast carcinoma tissue; A549 cells
  • General notes

     

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration. PubMed: 27252533
WB 1/10000 - 1/50000. Predicted molecular weight: 57 kDa.
IHC-P 1/100 - 1/250. Perform antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/2000.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA.
    • Sequence similarities

      Contains 4 RRM (RNA recognition motif) domains.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • 57 kDa RNA binding protein PPTB 1 antibody
      • 57 kDa RNA-binding protein PPTB-1 antibody
      • Heterogeneous nuclear ribonucleoprotein I antibody
      • Heterogeneous Nuclear Ribonucleoprotein Polypeptide I antibody
      • hnRNP I antibody
      • HNRNP-I antibody
      • HNRNPI antibody
      • HNRPI antibody
      • MGC10830 antibody
      • MGC8461 antibody
      • Polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I) antibody
      • Polypyrimidine Tract Binding Protein 1 antibody
      • Polypyrimidine tract binding protein antibody
      • Polypyrimidine tract-binding protein 1 antibody
      • pPTB antibody
      • PTB 1 antibody
      • PTB 2 antibody
      • PTB 3 antibody
      • PTB 4 antibody
      • PTB antibody
      • PTB T antibody
      • PTB1 antibody
      • PTB2 antibody
      • PTB3 antibody
      • PTB4 antibody
      • PTBP 1 antibody
      • PTBP1 antibody
      • PTBP1_HUMAN antibody
      • PTBT antibody
      • RNA Binding Protein antibody
      see all

    Images

    • All lanes : Anti-PTBP1 antibody [EPR9048(B)] (ab133734) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : PTBP1 knockout HAP1 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 57 kDa



      Lanes 1 - 2: Merged signal (red and green). Green - ab133734 observed at 57 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab133734 was shown to recognize PTBP1 in wild-type HAP1 cells as signal was lost at the expected MW in PTBP1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PTBP1 knockout samples were subjected to SDS-PAGE. Ab133734 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemical analysis of PTBP1 in paraffin embedded Human breast carcinoma tissue stained with ab133734 at a 1/100 dilution.
    • All lanes : Anti-PTBP1 antibody [EPR9048(B)] (ab133734) at 1/10000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : Daudi cell lysate
      Lane 4 : A549 cell lysate
      Lane 5 : BxPC3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 57 kDa

    • Immunofluorescent staining of PTBP1 in A549 cells, using ab133734 at a 1/250 dilution.
    • Flow cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling PTBP1 (red) with purified ab133734 at a 1/2000 dilution (1ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

    References

    This product has been referenced in:

    • Urdaneta EC  et al. Purification of cross-linked RNA-protein complexes by phenol-toluol extraction. Nat Commun 10:990 (2019). Read more (PubMed: 30824702) »
    • Dumbovic G  et al. A novel long non-coding RNA from NBL2 pericentromeric macrosatellite forms a perinucleolar aggregate structure in colon cancer. Nucleic Acids Res 46:5504-5524 (2018). Read more (PubMed: 29912433) »
    See all 4 Publications for this product

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