Product nameAnti-PTBP1 antibody [EPR9048(B)]
See all PTBP1 primary antibodies
DescriptionRabbit monoclonal [EPR9048(B)] to PTBP1
Tested applicationsSuitable for: ChIP, WB, IHC-P, Flow Cyt, ICC/IFmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to a region within Human PTBP1 (UniProt ID: P26599).
- Human fetal liver, Jurkat cell, Daudi cell, A549 and BxPC3 cell lysates; Human breast carcinoma tissue; A549 cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration. PubMed: 27252533|
|WB||1/10000 - 1/50000. Predicted molecular weight: 57 kDa.|
|IHC-P||1/100 - 1/250. Perform antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/250 - 1/500.|
FunctionPlays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA.
Sequence similaritiesContains 4 RRM (RNA recognition motif) domains.
- Information by UniProt
- 57 kDa RNA binding protein PPTB 1 antibody
- 57 kDa RNA-binding protein PPTB-1 antibody
- Heterogeneous nuclear ribonucleoprotein I antibody
All lanes : Anti-PTBP1 antibody [EPR9048(B)] (ab133734) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : PTBP1 knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 57 kDa
Lanes 1 - 2: Merged signal (red and green). Green - ab133734 observed at 57 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab133734 was shown to recognize PTBP1 in wild-type HAP1 cells as signal was lost at the expected MW in PTBP1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PTBP1 knockout samples were subjected to SDS-PAGE. Ab133734 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunohistochemical analysis of PTBP1 in paraffin embedded Human breast carcinoma tissue stained with ab133734 at a 1/100 dilution.
All lanes : Anti-PTBP1 antibody [EPR9048(B)] (ab133734) at 1/10000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Daudi cell lysate
Lane 4 : A549 cell lysate
Lane 5 : BxPC3 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 57 kDa
Immunofluorescent staining of PTBP1 in A549 cells, using ab133734 at a 1/250 dilution.
Flow cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling PTBP1 (red) with purified ab133734 at a 1/2000 dilution (1ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
This product has been referenced in:
- Urdaneta EC et al. Purification of cross-linked RNA-protein complexes by phenol-toluol extraction. Nat Commun 10:990 (2019). Read more (PubMed: 30824702) »
- Dumbovic G et al. A novel long non-coding RNA from NBL2 pericentromeric macrosatellite forms a perinucleolar aggregate structure in colon cancer. Nucleic Acids Res 46:5504-5524 (2018). Read more (PubMed: 29912433) »