Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-PTBP1 antibody [EPR9048(B)] - BSA and Azide free (ab240079)

Overview

  • Product name

    Anti-PTBP1 antibody [EPR9048(B)] - BSA and Azide free
    See all PTBP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR9048(B)] to PTBP1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cyt, ChIP, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PTBP1 aa 1-100. The exact sequence is proprietary.

  • General notes

    Ab240079 is the carrier-free version of ab133734. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240079 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240079 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Perform antigen retrieval before commencing with IHC staining protocol. 

 

ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration. PubMed: 27252533
WB Use at an assay dependent concentration. Predicted molecular weight: 57 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA.
    • Sequence similarities

      Contains 4 RRM (RNA recognition motif) domains.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • 57 kDa RNA binding protein PPTB 1 antibody
      • 57 kDa RNA-binding protein PPTB-1 antibody
      • Heterogeneous nuclear ribonucleoprotein I antibody
      • Heterogeneous Nuclear Ribonucleoprotein Polypeptide I antibody
      • hnRNP I antibody
      • HNRNP-I antibody
      • HNRNPI antibody
      • HNRPI antibody
      • MGC10830 antibody
      • MGC8461 antibody
      • Polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I) antibody
      • Polypyrimidine Tract Binding Protein 1 antibody
      • Polypyrimidine tract binding protein antibody
      • Polypyrimidine tract-binding protein 1 antibody
      • pPTB antibody
      • PTB 1 antibody
      • PTB 2 antibody
      • PTB 3 antibody
      • PTB 4 antibody
      • PTB antibody
      • PTB T antibody
      • PTB1 antibody
      • PTB2 antibody
      • PTB3 antibody
      • PTB4 antibody
      • PTBP 1 antibody
      • PTBP1 antibody
      • PTBP1_HUMAN antibody
      • PTBT antibody
      • RNA Binding Protein antibody
      see all

    Images

    • All lanes : Anti-PTBP1 antibody [EPR9048(B)] (ab133734) at 1/10000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : PTBP1 knockout HAP1 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 57 kDa



      Lanes 1 - 2: Merged signal (red and green). Green - ab133734 observed at 57 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab133734 was shown to recognize PTBP1 in wild-type HAP1 cells as signal was lost at the expected MW in PTBP1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PTBP1 knockout samples were subjected to SDS-PAGE. Ab133734 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133734).

    • Flow cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling PTBP1 (red) with purified ab133734 at a 1/2000 dilution (1ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133734).

    • Immunohistochemical analysis of PTBP1 in paraffin embedded Human breast carcinoma tissue stained with ab133734 at a 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133734).

    • Immunofluorescent staining of PTBP1 in A549 cells, using ab133734 at a 1/250 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133734).

    References

    ab240079 has not yet been referenced specifically in any publications.

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