Overview

  • Product name

    Anti-PTBP1 antibody [EPR9049(B)]
    See all PTBP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR9049(B)] to PTBP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PTBP1 aa 350-450. The exact sequence is proprietary.

  • Positive control

    • HeLa cells; Daudi, HeLa, Jurkat and BXPC3 cell lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA.
    • Sequence similarities

      Contains 4 RRM (RNA recognition motif) domains.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • 57 kDa RNA binding protein PPTB 1 antibody
      • 57 kDa RNA-binding protein PPTB-1 antibody
      • Heterogeneous nuclear ribonucleoprotein I antibody
      • Heterogeneous Nuclear Ribonucleoprotein Polypeptide I antibody
      • hnRNP I antibody
      • HNRNP-I antibody
      • HNRNPI antibody
      • HNRPI antibody
      • MGC10830 antibody
      • MGC8461 antibody
      • Polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I) antibody
      • Polypyrimidine Tract Binding Protein 1 antibody
      • Polypyrimidine tract binding protein antibody
      • Polypyrimidine tract-binding protein 1 antibody
      • pPTB antibody
      • PTB 1 antibody
      • PTB 2 antibody
      • PTB 3 antibody
      • PTB 4 antibody
      • PTB antibody
      • PTB T antibody
      • PTB1 antibody
      • PTB2 antibody
      • PTB3 antibody
      • PTB4 antibody
      • PTBP 1 antibody
      • PTBP1 antibody
      • PTBP1_HUMAN antibody
      • PTBT antibody
      • RNA Binding Protein antibody
      see all

    Images

    • All lanes : Anti-PTBP1 antibody [EPR9049(B)] (ab134950) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : PTBP1 knockout HAP1 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 57 kDa



      Lanes 1 - 2: Merged signal (red and green). Green - ab134950 observed at 57 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab134950 was shown to specifically react with PTBP1 in wild-type HAP1 cells as signal was lost in PTBP1 knockout cells. Wild-type and PTBP1 knockout samples were subjected to SDS-PAGE. ab134950 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Immunofluorescent analysis of HeLa cells labelling PTBP1 with ab134950 at 1/100 dilution.
    • All lanes : Anti-PTBP1 antibody [EPR9049(B)] (ab134950) at 1/1000 dilution

      Lane 1 : Daudi cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : BXPC3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 57 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab134950 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab134950.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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