Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9049(B)] to PTBP1 - BSA and Azide free
- Suitable for: ICC/IF, WB
- Knockout validated
- Reacts with: Human
Product nameAnti-PTBP1 antibody [EPR9049(B)] - BSA and Azide free
See all PTBP1 primary antibodies
DescriptionRabbit monoclonal [EPR9049(B)] to PTBP1 - BSA and Azide free
Tested applicationsSuitable for: ICC/IF, WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human PTBP1 aa 350-450. The exact sequence is proprietary.
ab248749 is the carrier-free version of ab134950 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248749 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Dissociation constant (KD)KD = 8.10 x 10 -11 M Learn more about KD
Storage bufferConstituent: PBS
Concentration information loading...
KO cell lysates
Our Abpromise guarantee covers the use of ab248749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 57 kDa.|
FunctionPlays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA.
Sequence similaritiesContains 4 RRM (RNA recognition motif) domains.
- Information by UniProt
- 57 kDa RNA binding protein PPTB 1 antibody
- 57 kDa RNA-binding protein PPTB-1 antibody
- Heterogeneous nuclear ribonucleoprotein I antibody
All lanes : Anti-PTBP1 antibody [EPR9049(B)] (ab134950) at 1/1000 dilution
Lane 1 : Wild-type HeLa lysate
Lane 2 : PTBP1 knockout HeLa lysate
Lane 3 : Wild-type HAP1 lysate
Lane 4 : PTBP1 knockout HAP1 lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 57 kDa
This data was developed using the same antibody clone in a different buffer formulation (ab134950).
ab134950 Anti-PTBP1 antibody [EPR9049(B)] was shown to specifically react with PTBP1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265155 (knockout cell lysate ab257614) was used. Wild-type and PTBP1 knockout samples were subjected to SDS-PAGE. ab134950 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab248749 has not yet been referenced specifically in any publications.