Anti-PTBP2 antibody (ab228930)
Key features and details
- Rabbit polyclonal to PTBP2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-PTBP2 antibody
See all PTBP2 primary antibodies -
Description
Rabbit polyclonal to PTBP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human PTBP2 (internal sequence). The exact sequence is proprietary.
Database link: Q9UKA9 -
Positive control
- IHC-P: Mouse muscle and hindbrain tissue. Rat hindbrain tissue. WB: Mouse eye tissue extracts. A549, NCI-H129 and HCT 116 whole cell lysate. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228930 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 57 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 57 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
RNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. May antagonize in a tissue-specific manner the ability of NOVA1 to activate exon selection. Beside its function in pre-mRNA splicing, plays also a role in the regulation of translation. Isoform 5 has a reduced affinity for RNA. -
Tissue specificity
Mainly expressed in brain although also detected in other tissues like heart and skeletal muscle. Isoform 1 and isoform 2 are specifically expressed in neuronal tissues. Isoform 3 and isoform 4 are expressed in non-neuronal tissues. Isoform 5 and isoform 6 are truncated forms expressed in non-neuronal tissues. -
Sequence similarities
Contains 4 RRM (RNA recognition motif) domains. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 58155 Human
- Entrez Gene: 56195 Mouse
- Entrez Gene: 310820 Rat
- Omim: 608449 Human
- SwissProt: Q9UKA9 Human
- SwissProt: Q91Z31 Mouse
- SwissProt: Q66H20 Rat
- Unigene: 269895 Human
see all -
Alternative names
- brPTB antibody
- FLJ34897 antibody
- MIBP antibody
see all
Images
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All lanes : Anti-PTBP2 antibody (ab228930) at 1/2000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
Lane 3 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 57 kDa7.5% SDS-PAGE
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PTBP2 (green) using ab228930 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain: Hoechst 33342 (blue), phalloidin cytoskeletal marker (red).
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Paraffin-embedded rat hindbrain tissue stained for PTBP2 with ab228930 at a 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded mouse muscle tissue stained for PTBP2 with ab228930 at a 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded mouse hindbrain tissue stained for PTBP2 with ab228930 at a 1/500 dilution in immunohistochemical analysis.
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Anti-PTBP2 antibody (ab228930) at 1/1000 dilution + Mouse eye tissue extracts at 50 µg
Developed using the ECL technique.
Predicted band size: 57 kDa10% SDS-PAGE
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab228930 has not yet been referenced specifically in any publications.