RecombinantRabMAb

Recombinant Anti-PTBP2 antibody [EPR9890] (ab154787)

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Western blot - Anti-PTBP2 antibody [EPR9890] (ab154787)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP2 antibody [EPR9890] (ab154787)
  • Immunocytochemistry/ Immunofluorescence - Anti-PTBP2 antibody [EPR9890] (ab154787)
  • Anti-PTBP2 antibody [EPR9890] (ab154787)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9890] to PTBP2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PTBP2 antibody [EPR9890]
    See all PTBP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR9890] to PTBP2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    within Human PTBP2 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • C6, Neuro-2a, SH-SY5Y, Y79 and HeLa whole cell lysate (ab150035); Human glioma tissue; HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab154787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      RNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. May antagonize in a tissue-specific manner the ability of NOVA1 to activate exon selection. Beside its function in pre-mRNA splicing, plays also a role in the regulation of translation. Isoform 5 has a reduced affinity for RNA.

    • Tissue specificity

      Mainly expressed in brain although also detected in other tissues like heart and skeletal muscle. Isoform 1 and isoform 2 are specifically expressed in neuronal tissues. Isoform 3 and isoform 4 are expressed in non-neuronal tissues. Isoform 5 and isoform 6 are truncated forms expressed in non-neuronal tissues.

    • Sequence similarities

      Contains 4 RRM (RNA recognition motif) domains.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • brPTB antibody
      • FLJ34897 antibody
      • MIBP antibody
      • Neural polypyrimidine tract binding protein antibody
      • Neural polypyrimidine tract-binding protein antibody
      • Neurally enriched homolog of PTB antibody
      • Neurally-enriched homolog of PTB antibody
      • nPTB 5 antibody
      • nPTB 6 antibody
      • nPTB 7 antibody
      • nPTB 8 antibody
      • nPTB antibody
      • nPTB5 antibody
      • nPTB6 antibody
      • nPTB7 antibody
      • nPTB8 antibody
      • Polypyrimidine tract binding protein 2 antibody
      • Polypyrimidine tract-binding protein 2 antibody
      • PTB antibody
      • PTB like antibody
      • PTB like protein antibody
      • PTB-like protein antibody
      • PTBLP antibody
      • PTBP 2 antibody
      • Ptbp2 antibody
      • PTBP2_HUMAN antibody
      • Splicing regulator antibody
      see all

    Images

    • Western blot - Anti-PTBP2 antibody [EPR9890] (ab154787)
      Western blot - Anti-PTBP2 antibody [EPR9890] (ab154787)
      All lanes : Anti-PTBP2 antibody [EPR9890] (ab154787) at 1/1000 dilution

      Lane 1 : C6 cell lysate
      Lane 2 : Neuro-2a cell lysate
      Lane 3 : SH-SY5Y cell lysate
      Lane 4 : Y79 cell lysate
      Lane 5 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 57 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP2 antibody [EPR9890] (ab154787)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP2 antibody [EPR9890] (ab154787)

      Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling PTBP2 with ab154787 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-PTBP2 antibody [EPR9890] (ab154787)
      Immunocytochemistry/ Immunofluorescence - Anti-PTBP2 antibody [EPR9890] (ab154787)
      Immunofluorescent analysis of HeLa cells labeling PTBP2 with ab154787 at 1/250 dilution.
    • Anti-PTBP2 antibody [EPR9890] (ab154787)
      Anti-PTBP2 antibody [EPR9890] (ab154787)

    References (2)

    Publishing research using ab154787? Please let us know so that we can cite the reference in this datasheet.

    ab154787 has been referenced in 2 publications.

    • Fukuoka M  et al. Supplemental Treatment for Huntington's Disease with miR-132 that Is Deficient in Huntington's Disease Brain. Mol Ther Nucleic Acids 11:79-90 (2018). PubMed: 29858092
    • Liu W  et al. Hepatitis B virus core protein inhibits Fas-mediated apoptosis of hepatoma cells via regulation of mFas/FasL and sFas expression. FASEB J 29:1113-23 (2015). PubMed: 25466893

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