Recombinant
RabMAb

Recombinant Anti-PTBP2 antibody [EPR9890] (ab154787)

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Overview

  • Product name

    Anti-PTBP2 antibody [EPR9890]
    See all PTBP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR9890] to PTBP2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    within Human PTBP2 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • C6, Neuro-2a, SH-SY5Y, Y79 and HeLa whole cell lysate (ab150035); Human glioma tissue; HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab154787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      RNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. May antagonize in a tissue-specific manner the ability of NOVA1 to activate exon selection. Beside its function in pre-mRNA splicing, plays also a role in the regulation of translation. Isoform 5 has a reduced affinity for RNA.

    • Tissue specificity

      Mainly expressed in brain although also detected in other tissues like heart and skeletal muscle. Isoform 1 and isoform 2 are specifically expressed in neuronal tissues. Isoform 3 and isoform 4 are expressed in non-neuronal tissues. Isoform 5 and isoform 6 are truncated forms expressed in non-neuronal tissues.

    • Sequence similarities

      Contains 4 RRM (RNA recognition motif) domains.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • brPTB antibody
      • FLJ34897 antibody
      • MIBP antibody
      • Neural polypyrimidine tract binding protein antibody
      • Neural polypyrimidine tract-binding protein antibody
      • Neurally enriched homolog of PTB antibody
      • Neurally-enriched homolog of PTB antibody
      • nPTB 5 antibody
      • nPTB 6 antibody
      • nPTB 7 antibody
      • nPTB 8 antibody
      • nPTB antibody
      • nPTB5 antibody
      • nPTB6 antibody
      • nPTB7 antibody
      • nPTB8 antibody
      • Polypyrimidine tract binding protein 2 antibody
      • Polypyrimidine tract-binding protein 2 antibody
      • PTB antibody
      • PTB like antibody
      • PTB like protein antibody
      • PTB-like protein antibody
      • PTBLP antibody
      • PTBP 2 antibody
      • Ptbp2 antibody
      • PTBP2_HUMAN antibody
      • Splicing regulator antibody
      see all

    Images

    • Western blot - Anti-PTBP2 antibody [EPR9890] (ab154787)
      Western blot - Anti-PTBP2 antibody [EPR9890] (ab154787)
      All lanes : Anti-PTBP2 antibody [EPR9890] (ab154787) at 1/1000 dilution

      Lane 1 : C6 cell lysate
      Lane 2 : Neuro-2a cell lysate
      Lane 3 : SH-SY5Y cell lysate
      Lane 4 : Y79 cell lysate
      Lane 5 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 57 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP2 antibody [EPR9890] (ab154787)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP2 antibody [EPR9890] (ab154787)

      Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling PTBP2 with ab154787 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-PTBP2 antibody [EPR9890] (ab154787)
      Immunocytochemistry/ Immunofluorescence - Anti-PTBP2 antibody [EPR9890] (ab154787)
      Immunofluorescent analysis of HeLa cells labeling PTBP2 with ab154787 at 1/250 dilution.

    References

    This product has been referenced in:

    • Fukuoka M  et al. Supplemental Treatment for Huntington's Disease with miR-132 that Is Deficient in Huntington's Disease Brain. Mol Ther Nucleic Acids 11:79-90 (2018). Read more (PubMed: 29858092) »
    • Liu W  et al. Hepatitis B virus core protein inhibits Fas-mediated apoptosis of hepatoma cells via regulation of mFas/FasL and sFas expression. FASEB J 29:1113-23 (2015). Read more (PubMed: 25466893) »
    See all 2 Publications for this product

    Publishing research using ab154787? Please let us know so that we can cite the reference in this datasheet.

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