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Rabbit monoclonal [EPR9890] to PTBP2
ICC/IF more details Unsuitable for:
Flow Cyt or IP
Mouse, Rat, Human
Synthetic peptide corresponding to residues in Human PTBP2 (UniProt Q9UKA9).
C6, Neuro-2a, SH-SY5Y, Y79 and HeLa cell lysates; Human glioma tissue; HeLa cells.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20ºC.
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
1/100 - 1/250.
1/250 - 1/500.
Is unsuitable for Flow Cyt or IP.
RNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. May antagonize in a tissue-specific manner the ability of NOVA1 to activate exon selection. Beside its function in pre-mRNA splicing, plays also a role in the regulation of translation. Isoform 5 has a reduced affinity for RNA.
Mainly expressed in brain although also detected in other tissues like heart and skeletal muscle. Isoform 1 and isoform 2 are specifically expressed in neuronal tissues. Isoform 3 and isoform 4 are expressed in non-neuronal tissues. Isoform 5 and isoform 6 are truncated forms expressed in non-neuronal tissues.
Contains 4 RRM (RNA recognition motif) domains.
Information by UniProt
Western blot - Anti-PTBP2 antibody [EPR9890] (ab154787)
All lanes : Anti-PTBP2 antibody [EPR9890] (ab154787) at 1/1000 dilution Lane 1 : C6 cell lysate Lane 2 : Neuro-2a cell lysate Lane 3 : SH-SY5Y cell lysate Lane 4 : Y79 cell lysate Lane 5 : HeLa cell lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : Goat anti-rabbit HRP at 1/2000 dilution Predicted band size: 57 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP2 antibody [EPR9890] (ab154787)
Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling PTBP2 with ab154787 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-PTBP2 antibody [EPR9890] (ab154787)
Immunofluorescent analysis of HeLa cells labeling PTBP2 with ab154787 at 1/250 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"