Anti-PTBP2 antibody [EPR9890] (Monoclonal) (ab154787)

Product name

Anti-PTBP2 antibody [EPR9890]
See all PTBP2 primary antibodies
Description

Rabbit monoclonal [EPR9890] to PTBP2
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide corresponding to residues in Human PTBP2 (UniProt Q9UKA9).
Positive control
- C6, Neuro-2a, SH-SY5Y, Y79 and HeLa cell lysates; Human glioma tissue; HeLa cells.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR9890
Isotype

IgG
Research areas

Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- HeLa whole cell lysate (ab150035)
- HeLa whole cell lysate (ab29545)

Our Abpromise guarantee covers the use of ab154787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
IHC-P		1/100 - 1/250.
ICC/IF		1/250 - 1/500.

Application notes

Is unsuitable for Flow Cyt or IP.

Function

RNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. May antagonize in a tissue-specific manner the ability of NOVA1 to activate exon selection. Beside its function in pre-mRNA splicing, plays also a role in the regulation of translation. Isoform 5 has a reduced affinity for RNA.
Tissue specificity

Mainly expressed in brain although also detected in other tissues like heart and skeletal muscle. Isoform 1 and isoform 2 are specifically expressed in neuronal tissues. Isoform 3 and isoform 4 are expressed in non-neuronal tissues. Isoform 5 and isoform 6 are truncated forms expressed in non-neuronal tissues.
Sequence similarities

Contains 4 RRM (RNA recognition motif) domains.
Cellular localization

Nucleus.
Target information above from: UniProt accession Q9UKA9 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 58155 Human
- Entrez Gene: 56195 Mouse
- Entrez Gene: 310820 Rat
- Omim: 608449 Human
- SwissProt: Q9UKA9 Human
- SwissProt: Q91Z31 Mouse
- SwissProt: Q66H20 Rat
- Unigene: 269895 Human
- Unigene: 596061 Human
- Unigene: 29966 Mouse
- Unigene: 64916 Rat
see all
Alternative names
- brPTB antibody
- FLJ34897 antibody
- MIBP antibody
- Neural polypyrimidine tract binding protein antibody
- Neural polypyrimidine tract-binding protein antibody
- Neurally enriched homolog of PTB antibody
- Neurally-enriched homolog of PTB antibody
- nPTB 5 antibody
- nPTB 6 antibody
- nPTB 7 antibody
- nPTB 8 antibody
- nPTB antibody
- nPTB5 antibody
- nPTB6 antibody
- nPTB7 antibody
- nPTB8 antibody
- Polypyrimidine tract binding protein 2 antibody
- Polypyrimidine tract-binding protein 2 antibody
- PTB antibody
- PTB like antibody
- PTB like protein antibody
- PTB-like protein antibody
- PTBLP antibody
- PTBP 2 antibody
- Ptbp2 antibody
- PTBP2_HUMAN antibody
- Splicing regulator antibody
see all

Western blot - Anti-PTBP2 antibody [EPR9890] (ab154787)

All lanes : Anti-PTBP2 antibody [EPR9890] (ab154787) at 1/1000 dilution

Lane 1 : C6 cell lysate
Lane 2 : Neuro-2a cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : Y79 cell lysate
Lane 5 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 57 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTBP2 antibody [EPR9890] (ab154787)

Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling PTBP2 with ab154787 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-PTBP2 antibody [EPR9890] (ab154787)

Immunofluorescent analysis of HeLa cells labeling PTBP2 with ab154787 at 1/250 dilution.

Datasheet

Western blot protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols

This product has been referenced in:

Fukuoka M et al. Supplemental Treatment for Huntington's Disease with miR-132 that Is Deficient in Huntington's Disease Brain. Mol Ther Nucleic Acids 11:79-90 (2018). Read more (PubMed: 29858092) »
Liu W et al. Hepatitis B virus core protein inhibits Fas-mediated apoptosis of hepatoma cells via regulation of mFas/FasL and sFas expression. FASEB J 29:1113-23 (2015). Read more (PubMed: 25466893) »

See all 2 Publications for this product

Publishing research using ab154787? Please let us know so that we can cite the reference in this datasheet.

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Anti-PTBP2 antibody [EPR9890] (ab154787)

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