Product nameAnti-PTBP2 antibody [EPR9891]
See all PTBP2 primary antibodies
DescriptionRabbit monoclonal [EPR9891] to PTBP2
Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide, corresponding to residues in Human PTBP2 (UniProt: Q9UKA9).
- Human fetal brain, Y79 and Neuro 2a lysates; Y79 cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab154853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 57 kDa.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionRNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. May antagonize in a tissue-specific manner the ability of NOVA1 to activate exon selection. Beside its function in pre-mRNA splicing, plays also a role in the regulation of translation. Isoform 5 has a reduced affinity for RNA.
Tissue specificityMainly expressed in brain although also detected in other tissues like heart and skeletal muscle. Isoform 1 and isoform 2 are specifically expressed in neuronal tissues. Isoform 3 and isoform 4 are expressed in non-neuronal tissues. Isoform 5 and isoform 6 are truncated forms expressed in non-neuronal tissues.
Sequence similaritiesContains 4 RRM (RNA recognition motif) domains.
- Information by UniProt
- brPTB antibody
- FLJ34897 antibody
- MIBP antibody
All lanes : Anti-PTBP2 antibody [EPR9891] (ab154853) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Y79 cell lysate
Lane 3 : Neuro 2a cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 57 kDa
Flow cytometric analysis of permeabilized Y79 cells labeling PTBP2 with ab154853 at 1/100 dilution (red). Rabbit IgG negative control (green).