Overview

  • Product name
    Anti-PTBP2 antibody [EPR9891]
    See all PTBP2 primary antibodies
  • Description
    Rabbit monoclonal [EPR9891] to PTBP2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human PTBP2 (UniProt: Q9UKA9).

  • Positive control
    • Human fetal brain, Y79 and Neuro 2a lysates; Y79 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function
      RNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. May antagonize in a tissue-specific manner the ability of NOVA1 to activate exon selection. Beside its function in pre-mRNA splicing, plays also a role in the regulation of translation. Isoform 5 has a reduced affinity for RNA.
    • Tissue specificity
      Mainly expressed in brain although also detected in other tissues like heart and skeletal muscle. Isoform 1 and isoform 2 are specifically expressed in neuronal tissues. Isoform 3 and isoform 4 are expressed in non-neuronal tissues. Isoform 5 and isoform 6 are truncated forms expressed in non-neuronal tissues.
    • Sequence similarities
      Contains 4 RRM (RNA recognition motif) domains.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • brPTB antibody
      • FLJ34897 antibody
      • MIBP antibody
      • Neural polypyrimidine tract binding protein antibody
      • Neural polypyrimidine tract-binding protein antibody
      • Neurally enriched homolog of PTB antibody
      • Neurally-enriched homolog of PTB antibody
      • nPTB 5 antibody
      • nPTB 6 antibody
      • nPTB 7 antibody
      • nPTB 8 antibody
      • nPTB antibody
      • nPTB5 antibody
      • nPTB6 antibody
      • nPTB7 antibody
      • nPTB8 antibody
      • Polypyrimidine tract binding protein 2 antibody
      • Polypyrimidine tract-binding protein 2 antibody
      • PTB antibody
      • PTB like antibody
      • PTB like protein antibody
      • PTB-like protein antibody
      • PTBLP antibody
      • PTBP 2 antibody
      • Ptbp2 antibody
      • PTBP2_HUMAN antibody
      • Splicing regulator antibody
      see all

    Images

    • All lanes : Anti-PTBP2 antibody [EPR9891] (ab154853) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : Y79 cell lysate
      Lane 3 : Neuro 2a cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 57 kDa

    • Flow cytometric analysis of permeabilized Y79 cells labeling PTBP2 with ab154853 at 1/100 dilution (red). Rabbit IgG negative control (green).

    References

    This product has been referenced in:
    • Tahmasebi S  et al. Control of embryonic stem cell self-renewal and differentiation via coordinated alternative splicing and translation of YY2. Proc Natl Acad Sci U S A 113:12360-12367 (2016). Read more (PubMed: 27791185) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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