Product nameAnti-PTCD3 antibody
See all PTCD3 primary antibodies
DescriptionRabbit polyclonal to PTCD3
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
- WB: RT4 and U-251 MG cell lysates. IHC-P: Human cerebellum tissue. ICC/IF: U-2 OS cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab243800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.4 µg/ml. Predicted molecular weight: 79 kDa.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionMitochondrial RNA-binding protein that has a role in mitochondrial translation.
Tissue specificityAbundant in testes, skeletal muscle and heart tissue.
Sequence similaritiesBelongs to the PTCD3 family.
Contains 10 PPR (pentatricopeptide) repeats.
- Information by UniProt
- DKFZp666K071 antibody
- FLJ20758 antibody
- mitochondrial antibody
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for PTCD3 (green) using ab243800 at 4 µg/ml in ICC/IF.
All lanes : Anti-PTCD3 antibody (ab243800) at 0.4 µg/ml
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) cell lysate
Predicted band size: 79 kDa
Formalin-fixed, paraffin-embedded human cerebellum tissue stained for PTCD3 using ab243800 at 1/200 dilution in immunohistochemical analysis.
ab243800 has not yet been referenced specifically in any publications.