Key features and details
- Rabbit polyclonal to PTCH2
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PTCH2 antibody
See all PTCH2 primary antibodies
DescriptionRabbit polyclonal to PTCH2
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- IHC-P: Human brain tissue. ICC/IF: HEK-293 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab238338 in the following tested applications.
|IHC-P||1/200 - 1/500.|
|ICC/IF||1/50 - 1/200.|
FunctionMay have a role in epidermal development. May act as a receptor for Sonic hedgehog (SHH).
Involvement in diseaseDefects in PTCH2 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children. Although the majority of medulloblastomas occur sporadically, some manifest within familial cancer syndromes such as Turcot syndrome and basal cell nevus syndrome (Gorlin syndrome).
Defects in PTCH2 are a cause of sporadic basal cell carcinoma (BCC) [MIM:605462].
Sequence similaritiesBelongs to the patched family.
Contains 1 SSD (sterol-sensing) domain.
- Information by UniProt
- patched (Drosophila) homolog 2 antibody
- patched homolog 2 (Drosophila) antibody
- Protein patched homolog 2 antibody
Paraffin-embedded human brain tissue stained for PTCH2 using ab238338 at 1/200 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
HEK-293 (Human epithelial cell line from embryonic kidney) cells stained for PTCH2 (green) using ab238338 at 1/66 dilution in ICC/IF. Secondary antibody is an Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238338 has been referenced in 1 publication.
- Kim HS et al. Expression profile of sonic hedgehog signaling-related molecules in basal cell carcinoma. PLoS One 14:e0225511 (2019). PubMed: 31756206