Key features and details
- Goat polyclonal to PTCHD1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PTCHD1 antibody
DescriptionGoat polyclonal to PTCHD1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig
- Human Brain (Cerebellum) lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab109407 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab109407 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 101 kDa).
1 hour primary incubation is recommended for this product.
Tissue specificityWidely expressed, including in various regions of the brain with highest expression in the gray and white cerebellum, followed by the cerebellar vermis and the pituitary gland.
Sequence similaritiesBelongs to the patched family.
Contains 1 SSD (sterol-sensing) domain.
Cellular localizationCell membrane.
- Information by UniProt
- 9630036J22Rik antibody
- AI848149 antibody
- FLJ30296 antibody
Anti-PTCHD1 antibody (ab109407) at 0.5 µg/ml + Human Brain Cerebellum lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 101 kDa
An additional band of unknown identity was also consistently observed at 50kDa. This band was successfully blocked by incubation with the immunizing peptide.
ab109407 has not yet been referenced specifically in any publications.