Anti-PTCHD1 antibody (ab109407)
Key features and details
- Goat polyclonal to PTCHD1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PTCHD1 antibody -
Description
Goat polyclonal to PTCHD1 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig -
Immunogen
Synthetic peptide:
C-DFQKTSRVSER
, corresponding to internal sequence amino acids 260-270 of Human PTCHD1 (NP_775766.2). -
Positive control
- Human Brain (Cerebellum) lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab109407 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109407 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 101 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 101 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Tissue specificity
Widely expressed, including in various regions of the brain with highest expression in the gray and white cerebellum, followed by the cerebellar vermis and the pituitary gland. -
Sequence similarities
Belongs to the patched family.
Contains 1 SSD (sterol-sensing) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 427995 Chicken
- Entrez Gene: 536443 Cow
- Entrez Gene: 139411 Human
- Entrez Gene: 211612 Mouse
- Entrez Gene: 100151829 Pig
- Entrez Gene: 317517 Rat
- Omim: 300828 Human
- SwissProt: Q96NR3 Human
see all -
Alternative names
- 9630036J22Rik antibody
- AI848149 antibody
- FLJ30296 antibody
see all
Images
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Anti-PTCHD1 antibody (ab109407) at 0.5 µg/ml + Human Brain Cerebellum lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 101 kDa
An additional band of unknown identity was also consistently observed at 50kDa. This band was successfully blocked by incubation with the immunizing peptide.
Datasheets and documents
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Datasheet download
References (0)
ab109407 has not yet been referenced specifically in any publications.