Overview

  • Product name
    Anti-PTCHD3 antibody - C-terminal
  • Description
    Rabbit polyclonal to PTCHD3 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PTCHD3 aa 696-725 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q3KNS1

  • Positive control
    • MCF-7 cell lysate; Human testis tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab171144 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 87 kDa.

Target

  • Function
    May play a role in sperm development or sperm function.
  • Tissue specificity
    Expressed in germ cells of the testis (at protein level).
  • Sequence similarities
    Belongs to the patched family.
    Contains 1 SSD (sterol-sensing) domain.
  • Cellular localization
    Membrane. Localizes to the midpiece of the sperm tail.
  • Information by UniProt
  • Database links
  • Alternative names
    • Patched domain containing 3 antibody
    • patched domain containing protein 3 antibody
    • Patched domain-containing protein 3 antibody
    • patched related protein antibody
    • Patched-related protein antibody
    • Ptchd3 antibody
    • PTHD3_HUMAN antibody
    • PTR antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue labeling PTCHD3 with ab171144 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Anti-PTCHD3 antibody - C-terminal (ab171144) at 1/100 dilution + MCF-7 cell lysate at 35 µg

    Predicted band size: 87 kDa

References

ab171144 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab171144.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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