Recombinant
RabMAb

Recombinant Anti-PTDSS1 antibody [EPR8118(2)] (ab157222)

Overview

  • Product name

    Anti-PTDSS1 antibody [EPR8118(2)]
    See all PTDSS1 primary antibodies
  • Description

    Rabbit monoclonal [EPR8118(2)] to PTDSS1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human PTDSS1.

  • Positive control

    • Human fetal liver, Raw264.7, NIH 3T3, Human fetal brain, A549 and 293T lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8118(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab157222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 56 kDa.

The observed band size of ab157222 is 42kda, this is attributed to the high content of hydrophobic amino acids in PSS1 (PMID: 8898108 and PMID: 10938271).

IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Relevance

      Phosphatidylserine (PS) accounts for 5 to 10% of cell membrane phospholipids. In addition to its role as a structural component, PS is involved in cell signaling, blood coagulation, and apoptosis. PS is synthesized by a calcium dependent base exchange reaction catalyzed by PS synthases (EC 2.7.8.8), like PTDSS1, that exchange L-serine for the polar head group of phosphatidylcholine (PC) or phosphatidylethanolamine (PE) (Sturbois-Balcerzak et al., 2001 [PubMed 11084049]).
    • Cellular localization

      Membrane; Multi pass membrane protein.
    • Database links

    • Alternative names

      • Phosphatidylserine synthase 1 antibody
      • PSS1 antibody
      • PSSA antibody
      • PtdSer synthase 1 antibody
      • Serine exchange enzyme I antibody
      see all

    Images

    • All lanes : Anti-PTDSS1 antibody [EPR8118(2)] (ab157222) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : Raw264.7 cell lysate
      Lane 3 : NIH 3T3 cell lysate
      Lane 4 : Human fetal brain lysate
      Lane 5 : A549 cell lysate
      Lane 6 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 56 kDa



      The observed band size of ab157222 is 42kda, this is attributed to the high content of hydrophobic amino acids in PSS1 (PMID: 8898108 and PMID: 10938271).

    • Anti-PTDSS1 antibody [EPR8118(2)] (ab157222) at 1/1000 dilution + His-tagged human PTDSS1 (aa2-35-GGGGS-aa2-35-GGGGS-aa2-35) recombinant protein 15ng

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 56 kDa


      Exposure time: 1 second


      Observed band size: 17 kDa (Recombinant protein fragment)

      Blocking buffer and concentration 5% NFDM/TBST
      Diluting buffer and concentration 5% NFDM /TBST

    • Immunoprecipitation : 293T cell lysate immunoprecipitated with ab157222 at 1/10

      Western Blot: ab157222 at 1/1000 labeling PTDSS1 in immunoprecipitation pellet from 293T cell lysate

      Secondary Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size : 56 kDa

      The observed band size of ab157222 is 42kda, this is attributed to the high content of hydrophobic amino acids in PSS1 (PMID: 8898108 and PMID: 10938271).

    References

    ab157222 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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