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    pten-antibody-y184-ab32199.pdf

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Cell Biology Cell Cycle Kinases/Phosphatases Phosphatases
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-PTEN antibody [Y184] (ab32199)

  • Datasheet
  • SDS
Reviews (15)Q&A (2)References (222)

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Western blot - Anti-PTEN antibody [Y184] (ab32199)
  • Western blot - Anti-PTEN antibody [Y184] (ab32199)
  • Western blot - Anti-PTEN antibody [Y184] (ab32199)
  • Western blot - Anti-PTEN antibody [Y184] (ab32199)
  • Western blot - Anti-PTEN antibody [Y184] (ab32199)
  • Western blot - Anti-PTEN antibody [Y184] (ab32199)
  • Western blot - Anti-PTEN antibody [Y184] (ab32199)
  • Anti-PTEN antibody [Y184] (ab32199)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y184] to PTEN
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

HRP

You may also be interested in

Protein
Recombinant human PTEN protein (ab157087)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Primary
Product image
Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] - BSA and Azide free (ab215967)

View more associated products

Overview

  • Product name

    Anti-PTEN antibody [Y184]
    See all PTEN primary antibodies
  • Description

    Rabbit monoclonal [Y184] to PTEN
  • Host species

    Rabbit
  • Specificity

    A 42kDa band is seen for some samples in addition to 50-54kDa band- we do not know the specificity of this band. For example Rat kidney, heart, spleen have bands around 50kDa but rat PC-12 cells have single band at ~42kDa.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human PTEN aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
    (Peptide available as ab157804)

  • Positive control

    • WB: HAP1, MCF7 and HEK-293 cell lysates; Human brain lysate; Mouse primary bone marrow derived macrophage whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    Y184
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Phosphatases
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • Lipid Phosphatases
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Phosphatases
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • PTEN pathway
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Metabolic disorders
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • HRP Anti-PTEN antibody [Y184] (ab199545)
    • Anti-PTEN antibody [Y184] - Low endotoxin, Azide free (ab219361)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Immunizing Peptide (Blocking)

    • PTEN peptide (ab157804)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human PTEN protein (ab157087)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (10)
1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 47 kDa).Can be blocked with PTEN peptide (ab157804).

For unpurified, use 1/500.

Notes
WB
1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 47 kDa).Can be blocked with PTEN peptide (ab157804).

For unpurified, use 1/500.

Target

  • Function

    Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form induces less tumor suppressive ability. In motile cells, suppresses the formation of lateral pseudopods and thereby promotes cell polarization and directed movement.
    Isoform alpha: Functional kinase, like isoform 1 it antagonizes the PI3K-AKT/PKB signaling pathway. Plays a role in mitochondrial energetic metabolism by promoting COX activity and ATP production, via collaboration with isoform 1 in increasing protein levels of PINK1.
  • Tissue specificity

    Expressed at a relatively high level in all adult tissues, including heart, brain, placenta, lung, liver, muscle, kidney and pancreas.
  • Involvement in disease

    Cowden syndrome 1
    Lhermitte-Duclos disease
    Bannayan-Riley-Ruvalcaba syndrome
    Squamous cell carcinoma of the head and neck
    Endometrial cancer
    PTEN mutations are found in a subset of patients with Proteus syndrome, a genetically heterogeneous condition. The molecular diagnosis of PTEN mutation positive cases classifies Proteus syndrome patients as part of the PTEN hamartoma syndrome spectrum. As such, patients surviving the early years of Proteus syndrome are likely at a greater risk of developing malignancies.
    Glioma 2
    VACTERL association with hydrocephalus
    Prostate cancer
    Macrocephaly/autism syndrome
    A microdeletion of chromosome 10q23 involving BMPR1A and PTEN is a cause of chromosome 10q23 deletion syndrome, which shows overlapping features of the following three disorders: Bannayan-Zonana syndrome, Cowden disease and juvenile polyposis syndrome.
  • Sequence similarities

    Contains 1 C2 tensin-type domain.
    Contains 1 phosphatase tensin-type domain.
  • Domain

    The C2 domain binds phospholipid membranes in vitro in a Ca(2+)-independent manner; this binding is important for its tumor suppressor function.
  • Post-translational
    modifications

    Constitutively phosphorylated by CK2 under normal conditions. Phosphorylated in vitro by MAST1, MAST2, MAST3 and STK11. Phosphorylation results in an inhibited activity towards PIP3. Phosphorylation can both inhibit or promote PDZ-binding. Phosphorylation at Tyr-336 by FRK/PTK5 protects this protein from ubiquitin-mediated degradation probably by inhibiting its binding to NEDD4. Phosphorylation by ROCK1 is essential for its stability and activity. Phosphorylation by PLK3 promotes its stability and prevents its degradation by the proteasome.
    Monoubiquitinated; monoubiquitination is increased in presence of retinoic acid. Deubiquitinated by USP7; leading to its nuclear exclusion. Monoubiquitination of one of either Lys-13 and Lys-289 amino acid is sufficient to modulate PTEN compartmentalization. Ubiquitinated by XIAP/BIRC4.
  • Cellular localization

    Secreted. May be secreted via a classical signal peptide and reenter into cells with the help of a poly-Arg motif and Cytoplasm. Nucleus. Nucleus, PML body. Monoubiquitinated form is nuclear. Nonubiquitinated form is cytoplasmic. Colocalized with PML and USP7 in PML nuclear bodies. XIAP/BIRC4 promotes its nuclear localization.
  • Target information above from: UniProt accession P60484 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5728 Human
    • Entrez Gene: 19211 Mouse
    • Entrez Gene: 50557 Rat
    • Omim: 601728 Human
    • SwissProt: P60484 Human
    • SwissProt: O08586 Mouse
    • Unigene: 500466 Human
    • Unigene: 729457 Human
    • Unigene: 245395 Mouse
    see all
  • Alternative names

    • 10q23del antibody
    • BZS antibody
    • DEC antibody
    • GLM2 antibody
    • MGC11227 antibody
    • MHAM antibody
    • MMAC1 antibody
    • MMAC1 phosphatase and tensin homolog deleted on chromosome 10 antibody
    • Mutated in multiple advanced cancers 1 antibody
    • Phosphatase and tensin homolog antibody
    • Phosphatase and tensin like protein antibody
    • Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN antibody
    • Pten antibody
    • PTEN_HUMAN antibody
    • PTEN1 antibody
    • TEP1 antibody
    see all

Images

  • Western blot - Anti-PTEN antibody [Y184] (ab32199)
    Western blot - Anti-PTEN antibody [Y184] (ab32199)

    Lanes 1 and 5: PTEN knockout HAP1 cell lysate (20 µg)
    Lanes 2 and 6: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Green signal from target – ab32199 observed at 47 kDa
    Lanes 3 and 4: Red signal from loading control – ab8245 observed at 37 kDa
    Lanes 5 and 6: Merged (red and green) signal

    ab32199 was shown to specifically recognize PTEN in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when PTEN knockout samples were used. Wild-type and PTEN knockout samples were subjected to SDS-PAGE, ab32199 and ab8245 (loading control to GAPDH) were diluted to 1/500 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-PTEN antibody [Y184] (ab32199)
    Western blot - Anti-PTEN antibody [Y184] (ab32199)

    Lanes 1: PTEN knockout HAP1 cell lysate (20 µg)
    Lanes 2: Wild-type HAP1 cell lysate (20 µg)
    Green signal from target
    Red signal from loading control – ab8245 observed at 37 kDa

    This western blot image is a comparison between ab32199 and a competitor's top cited mouse monoclonal antibody.

  • Western blot - Anti-PTEN antibody [Y184] (ab32199)
    Western blot - Anti-PTEN antibody [Y184] (ab32199)
    All lanes : Anti-PTEN antibody [Y184] (ab32199) at 1/10000 dilution (purified)

    All lanes : Brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 47 kDa
    Observed band size: 54 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-PTEN antibody [Y184] (ab32199)
    Western blot - Anti-PTEN antibody [Y184] (ab32199)
    All lanes : Anti-PTEN antibody [Y184] (ab32199) at 1/10000 dilution (purified)

    Lane 1 : MCF7 whole cell lysate
    Lane 2 : HEK293 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 47 kDa
    Observed band size: 54 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-PTEN antibody [Y184] (ab32199)
    Western blot - Anti-PTEN antibody [Y184] (ab32199)
    Anti-PTEN antibody [Y184] (ab32199) at 1/500 dilution (Unpurified) + MCF7 cell lysate

    Predicted band size: 47 kDa
    Observed band size: 54 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-PTEN antibody [Y184] (ab32199)
    Western blot - Anti-PTEN antibody [Y184] (ab32199)This image is courtesy of an anonymous Abreview
    All lanes : Anti-PTEN antibody [Y184] (ab32199) at 1/500 dilution (Unpurified)

    All lanes : Mouse primary bone marrow derived macrophage whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 47 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Treatment:

    Lane 1 - control siRNA 24 hours

    Lane 2 - PTEN siRNA 24 hours

    Lane 3 - control siRNA 48 hours

    Lane 4 - PTEN siRNA 48 hours

    See Abreview

  • Western blot - Anti-PTEN antibody [Y184] (ab32199)
    Western blot - Anti-PTEN antibody [Y184] (ab32199)This image is courtesy of an anonymous Abreview.
    Anti-PTEN antibody [Y184] (ab32199) at 1/500 dilution + RBL-2H3 whole cell lysate

    Secondary
    Goat Anti-rabbit HRP at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 47 kDa

    See Abreview

  • Anti-PTEN antibody [Y184] (ab32199)
    Anti-PTEN antibody [Y184] (ab32199)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (222)

Publishing research using ab32199? Please let us know so that we can cite the reference in this datasheet.

ab32199 has been referenced in 222 publications.

  • Adusumilli VS  et al. ROS Dynamics Delineate Functional States of Hippocampal Neural Stem Cells and Link to Their Activity-Dependent Exit from Quiescence. Cell Stem Cell 28:300-314.e6 (2021). PubMed: 33275875
  • Shan G  et al. Downregulated exosomal microRNA-148b-3p in cancer associated fibroblasts enhance chemosensitivity of bladder cancer cells by downregulating the Wnt/ß-catenin pathway and upregulating PTEN. Cell Oncol (Dordr) 44:45-59 (2021). PubMed: 33423167
  • Feiner LK  et al. An aging and p53 related marker: HOXA5 promoter methylation negatively correlates with mRNA and protein expression in old age. Aging (Albany NY) 13:4831-4849 (2021). PubMed: 33547267
  • Wu C  et al. LncRNA WT1-AS/miR-494-3p Regulates Cell Proliferation, Apoptosis, Migration and Invasion via PTEN/PI3K/AKT Signaling Pathway in Non-Small Cell Lung Cancer. Onco Targets Ther 14:891-904 (2021). PubMed: 33603394
  • Zhang Z  et al. miR-139-5p promotes neovascularization in diabetic retinopathy by regulating the phosphatase and tensin homolog. Arch Pharm Res 44:205-218 (2021). PubMed: 33609236
View all Publications for this product

Customer reviews and Q&As

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1-10 of 15 Abreviews

Immunocytochemistry/ Immunofluorescence abreview for Anti-PTEN antibody [Y184]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (PC3 Prostate cancer cells over-expresing PTEN)
Permeabilization
Yes - 0.1% Triton X-100
Specification
PC3 Prostate cancer cells over-expresing PTEN
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Dimitra Kalamida

Verified customer

Submitted Feb 04 2019

Western blot abreview for Anti-PTEN antibody [Y184]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (DU145 Prostate cancer cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
DU145 Prostate cancer cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Dimitra Kalamida

Verified customer

Submitted Jul 30 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PTEN antibody [Y184]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Prostate)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Target Retrieval Solution low pH
Permeabilization
Yes - 0.1% Triton X-100
Specification
Prostate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Formalin
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Dimitra Kalamida

Verified customer

Submitted Jun 01 2018

Western blot abreview for Anti-PTEN antibody [Y184]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (COS7)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
COS7
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 20 2015

Western blot abreview for Anti-PTEN antibody [Y184]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Dog Cell lysate - whole cell (Madin-Darby canine kidney (MDCK) cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
Madin-Darby canine kidney (MDCK) cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 02 2015

Western blot abreview for Anti-PTEN antibody [Y184]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Cow Cell lysate - whole cell (Bovine aortic endothelial cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
Bovine aortic endothelial cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 25 2015

Western blot abreview for Anti-PTEN antibody [Y184]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (RBL-2H3 cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
RBL-2H3 cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 08 2015

Western blot abreview for Anti-PTEN antibody [Y184]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
HEK293T cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 15 2015

Western blot abreview for Anti-PTEN antibody [Y184]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (Primary bone marrow derived macrophage)
Specification
Primary bone marrow derived macrophage
Treatment
siRNA
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 28 2014

Western blot abreview for Anti-PTEN antibody [Y184]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Colon cell line)
Loading amount
80 µg
Specification
Colon cell line
Gel Running Conditions
Reduced Denaturing (3-8%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 29 2010

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