Product namePterostilbene, Antioxidant and antiapoptotic agent
DescriptionAntioxidant and antiapoptotic agent; analog of resveratrol
Biological descriptionAntioxidant and antiapoptotic agent; analog of resveratrol (ab120726). Antineoplastic properties in vitro and in vivo.
Storage instructionsStore at -20°C. Store In the Dark. Store under desiccating conditions. This product is air and light sensitive and impurities can occur as a result of air oxidation or due to metabolism by microbes.
Solubility overviewSoluble in DMSO to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Our Abpromise guarantee covers the use of ab120961 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab51031 staining beclin1 in MCF7 cells treated with pterostilbene (ab120961), by ICC/IF. Increase in beclin1 expression correlates with increased concentration of pterostilbene as described in literature.
The cells were incubated at 37°C for 48h in media containing different concentrations of ab120961 (pterostilbene) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab51031 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
This product has been referenced in:
- Zhang L et al. Pterostilbene protects vascular endothelial cells against oxidized low-density lipoprotein-induced apoptosis in vitro and in vivo. Apoptosis 17:25-36 (2012). Read more (PubMed: 21928089) »
- McCormack D & McFadden D Pterostilbene and cancer: current review. J Surg Res 173:e53-61 (2012). Read more (PubMed: 22099605) »
- Park ES et al. Pterostilbene, a natural dimethylated analog of resveratrol, inhibits rat aortic vascular smooth muscle cell proliferation by blocking Akt-dependent pathway. Vascul Pharmacol 53:61-7 (0). Read more (PubMed: 20398797) »