The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000. Detects a band of approximately 42 kDa (predicted molecular weight: 35 kDa).
Transcriptional activator. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays an important role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells (By similarity). Plays an important role in cerebellar development.
Pancreas-specific (at protein level). Loss of expression is seen in ductal type pancreas cancers.
Involvement in disease
Defects in PTF1A are the cause of diabetes mellitus and cerebellar hypoplasia/agenesis (DMCH) [MIM:609069].
Contains 1 basic helix-loop-helix (bHLH) domain.
Nucleus. Cytoplasm. In chronic pancreatitis associated with pancreas cancer preferentially accumulates in the cytoplasm of acinar/ductular complexes. In the cytoplasm loses its ability to form the PTF1 complex.
PTF1A is a pancreas specific transcription factor. Mammalian studies have implicated important roles for the basic helix-loop-helix transcription factor PTF1A-p48 in the development of both exocrine and endocrine pancreas.
bHLH transcription factor p48 antibody
Class A basic helix-loop-helix protein 29 antibody
Class II bHLH protein PTF1A antibody
Exocrine pancreas specific transcription factor p48 antibody
p48 DNA binding subunit of transcription factor PTF1 antibody
p48 DNA-binding subunit of transcription factor PTF1 antibody
Pancreas specific transcription factor 1a antibody
The molecular weight observed is consistent with what has been described in the literature (PMID: 18347078).
Western blot - Anti-PTF1A antibody [EPR19011] (ab182398)
All lanes : Anti-PTF1A antibody [EPR19011] (ab182398) at 1/2000 dilution
Lane 1 : Rat heart lysate at 20 µg Lane 2 : Rat pancreas lysate at 20 µg Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate at 10 µg Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
PTF1A was immunoprecipitated from 1 mg of NIH/3T3 (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab182398 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab182398 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/10000 dilution.
Lane 1: NIH/3T3 whole cell lysate 10 μg (Input).
Lane 2: ab182398 IP in NIH/3T3 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab182398 in NIH/3T3 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.