Recombinant
RabMAb

Recombinant Anti-PTF1A antibody [EPR19011] - BSA and Azide free (ab224794)

Overview

  • Product name

    Anti-PTF1A antibody [EPR19011] - BSA and Azide free
    See all PTF1A primary antibodies
  • Description

    Rabbit monoclonal [EPR19011] to PTF1A - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein aa 1 to the C-terminus.
    Database link: Q9QX98

  • Positive control

    • WB: Human fetal heart and fetal kidney lysates; BxPC-3, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Rat heart and pancreas lysates; Mouse pancreas lysate. IP: NIH/3T3 whole cell lysate.
  • General notes

    Ab224794 is the carrier-free version of ab182398. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab224794 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab224794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 35 kDa).
IP Use at an assay dependent concentration.

Target

  • Function

    Transcriptional activator. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays an important role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells (By similarity). Plays an important role in cerebellar development.
  • Tissue specificity

    Pancreas-specific (at protein level). Loss of expression is seen in ductal type pancreas cancers.
  • Involvement in disease

    Defects in PTF1A are the cause of diabetes mellitus and cerebellar hypoplasia/agenesis (DMCH) [MIM:609069].
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization

    Nucleus. Cytoplasm. In chronic pancreatitis associated with pancreas cancer preferentially accumulates in the cytoplasm of acinar/ductular complexes. In the cytoplasm loses its ability to form the PTF1 complex.
  • Information by UniProt
  • Database links

  • Form

    PTF1A is a pancreas specific transcription factor. Mammalian studies have implicated important roles for the basic helix-loop-helix transcription factor PTF1A-p48 in the development of both exocrine and endocrine pancreas.
  • Alternative names

    • bHLH transcription factor p48 antibody
    • bHLHa29 antibody
    • Class A basic helix-loop-helix protein 29 antibody
    • Class II bHLH protein PTF1A antibody
    • Exocrine pancreas specific transcription factor p48 antibody
    • p48 DNA binding subunit of transcription factor PTF1 antibody
    • p48 DNA-binding subunit of transcription factor PTF1 antibody
    • PACA antibody
    • PAGEN2 antibody
    • Pancreas specific transcription factor 1a antibody
    • Pancreas transcription factor 1 subunit alpha antibody
    • Pancreas-specific transcription factor 1a antibody
    • PTF 1A antibody
    • PTF1 p48 antibody
    • PTF1-p48 antibody
    • Ptf1a antibody
    • PTF1A_HUMAN antibody
    • PTF1P48 antibody
    see all

References

ab224794 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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