• Product name
  • Description
    Rabbit polyclonal to PTGER3
  • Host species
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to PTGER3 (C terminal).
    Database link: NP_942010.1



Our Abpromise guarantee covers the use of ab21227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB 1/500. Predicted molecular weight: 43 kDa.


  • Function
    Receptor for prostaglandin E2 (PGE2); the EP3 receptor may be involved in inhibition of gastric acid secretion, modulation of neurotransmitter release in central and peripheral neurons, inhibition of sodium and water reabsorption in kidney tubulus and contraction in uterine smooth muscle. The activity of this receptor can couple to both the inhibition of adenylate cyclase mediated by G-I proteins, and to an elevation of intracellular calcium. The various isoforms have identical ligand binding properties but can interact with different second messenger systems.
  • Tissue specificity
    Expressed in small intestine, heart, pancreas, gastric fundic mucosa, mammary artery and pulmonary vessels.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EP 3 antibody
    • EP3 antibody
    • EP3 I antibody
    • EP3 II antibody
    • EP3 III antibody
    • EP3 IV antibody
    • Ep3 prostanoid receptor antibody
    • Ep3 receptor antibody
    • EP3 subtype antibody
    • EP3e antibody
    • HGNC:9595 antibody
    • MGC141828 antibody
    • MGC141829 antibody
    • MGC27302 antibody
    • PE2R3_HUMAN antibody
    • PGE receptor EP3 subtype antibody
    • PGE2 R antibody
    • PGE2 receptor EP3 subtype antibody
    • PGE2-R antibody
    • PGE2R antibody
    • Pgerep3 antibody
    • Prostaglandin E receptor 3 (subtype EP3) antibody
    • Prostaglandin E receptor 3 antibody
    • Prostaglandin E receptor EP3 subtype 3 isoform antibody
    • Prostaglandin E2 receptor antibody
    • Prostaglandin e2 receptor ep3 antibody
    • Prostaglandin E2 receptor EP3 subtype antibody
    • Prostaglandin receptor (PGE 2) antibody
    • Prostaglandin receptor 3, EP3, subtype antibody
    • Prostanoid EP3 receptor antibody
    • PTGER 3 antibody
    • Ptger3 antibody
    • Ptgerep3 antibody
    • Rep3 antibody
    • rEP3a antibody
    • rEP3b antibody
    see all


This product has been referenced in:
  • Mak S  et al. Differential expression of genes in the calcium-signaling pathway underlies lesion development in the LDb mouse model of atherosclerosis. Atherosclerosis 213:40-51 (2010). Read more (PubMed: 20667539) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


Thank you for sending on these further details. I apologise for the delay in responding to your message. I have been waiting to obtain more details on the immunogen for this antibody from the laboratory. I can confirm that the immunogen sequence was from amino acids 367-388 of EP3. This corresponds to cytoplasmic C-terminal end of the protein. I can therefore recommend adding a permeabilisation step to your staining procedure. As it is a membrane protein, I can suggest using a mild detergent, such is digitonin or saponin at 1% (Triton will be too harsh). I can also recommend that as you are detecting a recombinant protein, which are sometimes shorter than full length protein, it is important to ensure this includes the immunogen sequence. I can suggest this can be checked with the immunogen details above. I can also strongly recommend using a positive control tissue (expressing EP3) which will indicate if the antibody is working correctly. I hope this information is helpful. Should you have any further difficulties, please do not hesitate to contact us again so we can investigate further.

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We have found only 38% homology between the sequence you provided and the immunizing peptide used to generate ab21227, we therefore do not think the antibody will recognize chicken EP3 receptor. Please do not hesitate to contact us again if you need further information,

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