Product nameAnti-PTGES2/Gbf1 antibody
See all PTGES2/Gbf1 primary antibodies
DescriptionRabbit polyclonal to PTGES2/Gbf1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Cynomolgus monkey
Recombinant fragment corresponding to Human PTGES2/Gbf1 aa 88-377.
ERSAAQLSLSSRLQLTLYQYKTCPFCSKVRAFLDFHALPYQVVEVNPVRR AEIKFSSYRKVPILVAQEGESSQQLNDSSVIISALKTYLVSGQPLEEIIT YYPAMKAVNEQGKEVTEFGNKYWLMLNEKEAQQVYGGKEARTEEMKWRQW ADDWLVHLISPNVYRTPTEALASFDYIVREGKFGAVEGAVAKYMGAAAMY LISKRLKSRHRLQDNVREDLYEAADKWVAAVGKDRPFMGGQKPNLADLAV YGVLRVMEGLDAFDDLMQHTHIQPWYLRVERAITEASPAH
Database link: Q9H7Z7
- WB: HEK-293T, HeLa and HepG2 whole cell lysate (ab7900). IHC-P: Human colon cancer and placenta tissues.
This product was previously labelled as PTGES2
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229961 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).|
FunctionIsomerase that catalyzes the conversion of unstable intermediate of prostaglandin E2 H2 (PGH2) into the more stable prostaglandin E2 (PGE2) form. May also have transactivation activity toward IFN-gamma (IFNG), possibly via an interaction with CEBPB; however, the relevance of transcription activation activity remains unclear.
Tissue specificityWidely expressed. Expressed in the heart, including apex, inter-ventricular septum, both atria and ventricles, but not in the aorta. Also expressed in fetal heart. Detected in various regions of the brain: cerebellum; occipital, frontal and parietal lobes. Also expressed in the lymph nodes, skeletal muscle, kidney and trachea, but not in the thymus or lung. Overexpressed in colorectal cancer.
PathwayLipid metabolism; prostaglandin biosynthesis.
Sequence similaritiesBelongs to the GST superfamily.
Contains 1 glutaredoxin domain.
Contains 1 GST C-terminal domain.
Cellular localizationGolgi apparatus membrane and Cytoplasm > perinuclear region. Synthetized as a Golgi membrane-bound protein, which is further cleaved into the predominant soluble truncated form. The truncated form is cytoplasmic and is enriched in the perinuclear region.
- Information by UniProt
- C9orf15 antibody
- FLJ14038 antibody
- Gamma interferon activated transcriptional element binding factor 1 antibody
All lanes : Anti-PTGES2/Gbf1 antibody (ab229961) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Paraffin-embedded human colon cancer tissue stained for PTGES2/Gbf1 using ab229961 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human placenta tissue stained for PTGES2/Gbf1 using ab229961 at 1/100 dilution in immunohistochemical analysis.
ab229961 has not yet been referenced specifically in any publications.