Key features and details
- Rabbit polyclonal to PTGES2/Gbf1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PTGES2/Gbf1 antibody
See all PTGES2/Gbf1 primary antibodies
DescriptionRabbit polyclonal to PTGES2/Gbf1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Cynomolgus monkey
Recombinant fragment corresponding to Human PTGES2/Gbf1 aa 88-377.
ERSAAQLSLSSRLQLTLYQYKTCPFCSKVRAFLDFHALPYQVVEVNPVRR AEIKFSSYRKVPILVAQEGESSQQLNDSSVIISALKTYLVSGQPLEEIIT YYPAMKAVNEQGKEVTEFGNKYWLMLNEKEAQQVYGGKEARTEEMKWRQW ADDWLVHLISPNVYRTPTEALASFDYIVREGKFGAVEGAVAKYMGAAAMY LISKRLKSRHRLQDNVREDLYEAADKWVAAVGKDRPFMGGQKPNLADLAV YGVLRVMEGLDAFDDLMQHTHIQPWYLRVERAITEASPAH
Database link: Q9H7Z7
- WB: HEK-293T, HeLa and HepG2 whole cell lysate (ab7900). IHC-P: Human colon cancer and placenta tissues.
This product was previously labelled as PTGES2
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229961 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).|
FunctionIsomerase that catalyzes the conversion of unstable intermediate of prostaglandin E2 H2 (PGH2) into the more stable prostaglandin E2 (PGE2) form. May also have transactivation activity toward IFN-gamma (IFNG), possibly via an interaction with CEBPB; however, the relevance of transcription activation activity remains unclear.
Tissue specificityWidely expressed. Expressed in the heart, including apex, inter-ventricular septum, both atria and ventricles, but not in the aorta. Also expressed in fetal heart. Detected in various regions of the brain: cerebellum; occipital, frontal and parietal lobes. Also expressed in the lymph nodes, skeletal muscle, kidney and trachea, but not in the thymus or lung. Overexpressed in colorectal cancer.
PathwayLipid metabolism; prostaglandin biosynthesis.
Sequence similaritiesBelongs to the GST superfamily.
Contains 1 glutaredoxin domain.
Contains 1 GST C-terminal domain.
Cellular localizationGolgi apparatus membrane and Cytoplasm > perinuclear region. Synthetized as a Golgi membrane-bound protein, which is further cleaved into the predominant soluble truncated form. The truncated form is cytoplasmic and is enriched in the perinuclear region.
- Information by UniProt
- C9orf15 antibody
- FLJ14038 antibody
- Gamma interferon activated transcriptional element binding factor 1 antibody
All lanes : Anti-PTGES2/Gbf1 antibody (ab229961) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Paraffin-embedded human colon cancer tissue stained for PTGES2/Gbf1 using ab229961 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human placenta tissue stained for PTGES2/Gbf1 using ab229961 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229961 has not yet been referenced specifically in any publications.