Key features and details
- Goat polyclonal to PTGIS/PGIS
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PTGIS/PGIS antibody
See all PTGIS/PGIS primary antibodies
DescriptionGoat polyclonal to PTGIS/PGIS
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
- WB: Human lung and ovary lysates.
This product was previously labelled as PTGIS
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab223578 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
Our Abpromise guarantee covers the use of ab223578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 57 kDa).
1 hour primary incubation is recommended for this product.
FunctionCatalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2).
Tissue specificityWidely expressed; particularly abundant in ovary, heart, skeletal muscle, lung and prostate.
Sequence similaritiesBelongs to the cytochrome P450 family.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- CYP8 antibody
- CYP8A1 antibody
- Cytochrome P450, family 8, subfamily A, polypeptide 1 antibody
All lanes : Anti-PTGIS/PGIS antibody (ab223578) at 0.5 µg/ml
Lane 1 : Human lung lysate (in RIPA buffer)
Lane 2 : Human ovary lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 57 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
ab223578 has not yet been referenced specifically in any publications.