Overview

  • Product name

  • Description

    Rabbit polyclonal to PTGIS/PGIS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, IHC-Fr, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Sheep, Cow, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse PTGIS/PGIS aa 475-490.
    Sequence:

    PEFDLSRYGFGLMQPE


    (Peptide available as ab187257)

  • General notes

     

     

     This product was previously labelled as PTGIS

     

Applications

Our Abpromise guarantee covers the use of ab23668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/250. Predicted molecular weight: 57 kDa.

Target

Images

  • ab 23668 at 1/200 staining primary mouse myoblasts by ICC/IF.

    The tissue section was formaldehyde fixed, and permeabilized and blocked with 100% TNB buffer.  The blocking time was 1 hour, at 25°C.

    The primary antibody was incubated for 16 hours at 4°C.

    The secondary antibody was a biotin conjugated Donkey anti-Rabbit IgG polyclonal diluted1/800.

    See Abreview

  • ab23668 (4µg/ml) staining PTGIS/PGIS in human adrenal gland using an automated system (DAKO Autostainer Plus). Using this protocol there is apical cytoplasmic/ endoplasmic reticulum staining.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References

This product has been referenced in:

  • Ebmeyer J  et al. Pyrrolizidine alkaloid-induced alterations of prostanoid synthesis in human endothelial cells. Chem Biol Interact 298:104-111 (2019). Read more (PubMed: 30465738) »
  • Russo A  et al. Differential Expression of Prostaglandin I2 Synthase Associated with Arachidonic Acid Pathway in the Oral Squamous Cell Carcinoma. J Oncol 2018:6301980 (2018). Read more (PubMed: 30532780) »
See all 7 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Pig Tissue lysate - whole (Carotid artery)
Gel Running Conditions
Reduced Non-Denaturing (Native) (10%)
Loading amount
30 µg
Specification
Carotid artery
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Apr 09 2008

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Primary mouse myoblasts)
Permeabilization
Yes - TNB buffer, Perkin Elmer
Specification
Primary mouse myoblasts
Blocking step
TNB buffer, Perkin Elmer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Nov 23 2007

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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