Overview

  • Product name

  • Description

    Rabbit polyclonal to PTHLH
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog, Pig
  • Immunogen

    Recombinant fragment containing a sequence corresponding to a region within amino acids 1-127 of Human PTHLH.(Uniprot: P12272)

  • Positive control

    • A549 cell lysate, HepG2 whole cell lysate (ab7900) and HTC116 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab125700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 20 kDa.

Target

  • Function

    Neuroendocrine peptide which is a critical regulator of cellular and organ growth, development, migration, differentiation and survival and of epithelial calcium ion transport. Regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. Required for skeletal homeostasis. Promotes mammary mesenchyme differentiation and bud outgrowth by modulating mesenchymal cell responsiveness to BMPs. Upregulates BMPR1A expression in the mammary mesenchyme and this increases the sensitivity of these cells to BMPs and allows them to respond to BMP4 in a paracrine and/or autocrine fashion. BMP4 signaling in the mesenchyme, in turn, triggers epithelial outgrowth and augments MSX2 expression, which causes the mammary mesenchyme to inhibit hair follicle formation within the nipple sheath (By similarity). Promotes colon cancer cell migration and invasion in an integrin alpha-6/beta-1-dependent manner through activation of Rac1.
    Osteostatin is a potent inhibitor of osteoclastic bone resorption.
  • Tissue specificity

    Ubiquitous. Also expressed in the mammary gland.
  • Involvement in disease

    Brachydactyly E2
  • Sequence similarities

    Belongs to the parathyroid hormone family.
  • Post-translational
    modifications

    There are 3 principal secretory forms, called PTHrP[1-36], PTHrP[38-94], and osteostatin (PTHrP[107-139]) arising from endoproteolytic cleavage of the initial translation product. Each of these secretory forms is believed to have one or more of its own receptors that mediates the normal paracrine, autocrine and endocrine actions.
  • Cellular localization

    Cytoplasm. Nucleus. Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • HHM antibody
    • MGC14611 antibody
    • Osteostatin antibody
    • Parathyroid hormon like hormone isoform 2 preproprotein antibody
    • Parathyroid hormone like hormone antibody
    • Parathyroid hormone like hormone isoform 1 preproprotein antibody
    • Parathyroid hormone like protein antibody
    • Parathyroid hormone like related protein antibody
    • Parathyroid hormone related protein antibody
    • Parathyroid hormone-like protein antibody
    • Parathyroid like protein antibody
    • PLP antibody
    • PTH related protein antibody
    • PTH-rP antibody
    • PTHLH antibody
    • PTHR antibody
    • PTHR_HUMAN antibody
    • PTHrP antibody
    • PTHrP[107-139] antibody
    see all

Images

  • All lanes : Anti-PTHLH antibody (ab125700) at 1/1000 dilution

    Lane 1 : HepG2 lysate
    Lane 2 : HCT116 lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 20 kDa

References

This product has been referenced in:

  • Duan Z & Lu J Involvement of Aryl Hydrocarbon Receptor in L-Kynurenine-Mediated Parathyroid Hormone-Related Peptide Expression. Horm Cancer N/A:N/A (2019). Read more (PubMed: 30689168) »
  • Cao X  et al. miR-195 contributes to human osteoarthritis via targeting PTHrP. J Bone Miner Metab N/A:N/A (2018). Read more (PubMed: 30465089) »
See all 2 Publications for this product

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Epithelial Breast Cells)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
50 µg
Specification
Epithelial Breast Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Jasmine Johnson

Verified customer

Submitted Dec 04 2018

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