Key features and details
- Mouse monoclonal [D1] to PTHLH
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG2a
Product nameAnti-PTHLH antibody [D1]
See all PTHLH primary antibodies
DescriptionMouse monoclonal [D1] to PTHLH
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse
Recombinant full length protein (His-tag) corresponding to Rat PTHLH aa 37-177. Two N-terminal tags, His-tag and S-tag (Expressed in E.coli).
AVSEHQLLHDKGKSIQDLRRRFFLHHLIAEIHTAEIRATSEVSPNSKPAP NTKNHPVRFGSDDEGRYLTQETNKVETYKEQPLKTPGKKKKGKPGKRREQ EKKKRRTRSAWPGTTGSGLLEDPQPHTSPTSTSLEPSSRTH
Database link: P13085
- IHC-P: Rat pancreas tissue. WB: Rat thymus lysate; Recombinant rat PTHLH protein.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from TCS.
Light chain typekappa
Our Abpromise guarantee covers the use of ab239527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 20 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionNeuroendocrine peptide which is a critical regulator of cellular and organ growth, development, migration, differentiation and survival and of epithelial calcium ion transport. Regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. Required for skeletal homeostasis. Promotes mammary mesenchyme differentiation and bud outgrowth by modulating mesenchymal cell responsiveness to BMPs. Upregulates BMPR1A expression in the mammary mesenchyme and this increases the sensitivity of these cells to BMPs and allows them to respond to BMP4 in a paracrine and/or autocrine fashion. BMP4 signaling in the mesenchyme, in turn, triggers epithelial outgrowth and augments MSX2 expression, which causes the mammary mesenchyme to inhibit hair follicle formation within the nipple sheath (By similarity). Promotes colon cancer cell migration and invasion in an integrin alpha-6/beta-1-dependent manner through activation of Rac1.
Osteostatin is a potent inhibitor of osteoclastic bone resorption.
Tissue specificityUbiquitous. Also expressed in the mammary gland.
Involvement in diseaseBrachydactyly E2
Sequence similaritiesBelongs to the parathyroid hormone family.
modificationsThere are 3 principal secretory forms, called PTHrP[1-36], PTHrP[38-94], and osteostatin (PTHrP[107-139]) arising from endoproteolytic cleavage of the initial translation product. Each of these secretory forms is believed to have one or more of its own receptors that mediates the normal paracrine, autocrine and endocrine actions.
Cellular localizationCytoplasm. Nucleus. Secreted.
- Information by UniProt
- HHM antibody
- MGC14611 antibody
- Osteostatin antibody
Paraffin-embedded rat pancreas tissue stained for PTHLH using ab239527 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Anti-PTHLH antibody [D1] (ab239527) at 6 µg/ml + Rat thymus lysate
Predicted band size: 20 kDa
Anti-PTHLH antibody [D1] (ab239527) at 2 µg/ml + Recombinant rat PTHLH protein
Predicted band size: 20 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab239527 has not yet been referenced specifically in any publications.