Product nameAnti-PTIP antibody
See all PTIP primary antibodies
DescriptionRabbit polyclonal to PTIP
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Pig, Chimpanzee, Orangutan
Synthetic peptide corresponding to Human PTIP (N terminal). Synthetic peptide mapping to a region between residues 1 and 50 of Human PTIP, using the numbering given in Jowsey, Doherty and Rouse, 2004, J. Biol. Chem. 279(53):55562-55569
Database link: Q6ZW49-6
- Whole cell lysate from 293T cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2500 - 1/25000. Detects a band of approximately 130 kDa (predicted molecular weight: 118 kDa).|
|IP||Use at 5-10 µg/mg of lysate.|
FunctionInvolved in DNA damage response and in transcriptional regulation through histone methyltransferase (HMT) complexes. Plays a role in early development. In DNA damage response is required for cell survival after ionizing radiation. In vitro shown to be involved in the homologous recombination mechanism for the repair of double-strand breaks (DSBs). Its localization to DNA damage foci requires RNF8 and UBE2N. Recruits TP53BP1 to DNA damage foci and, at least in particular repair processes, effective DNA damage response appears to require the association with TP53BP1 phosphorylated by ATM at 'Ser-25'. Together with TP53BP1 regulates ATM association. Recruits PA1 to sites of DNA damage and the PA1:PAXIP1 complex is required for cell survival in response to DNA damage; the function is probbaly independent of MLL-containing histone methyltransferase (HMT) complexes. Promotes ubiquitination of PCNA following UV irradiation and may regulate recruitment of polymerase eta and RAD51 to chromatin after DNA damage. Proposed to be involved in transcriptional regulation by linking MLL-containing histone methyltransferase (HMT) complexes to gene promoters by interacting with promoter-bound transcription factors such as PAX2. Associates with gene promoters that are known to be regulated by MLL2. During immunoglobulin class switching in activated B cells is involved in trimethylation of histone H3 at 'Lys-4' and in transcription initiation of downstream switch regions at the immunoglobulin heavy-chain (Igh) locus; this function appears to involve the recruitment of MLL-containing HMT complexes.
Sequence similaritiesContains 6 BRCT domains.
DomainThe BRCT 5 and 6 domains function as a single module and are necessary and sufficient for in vitro phospho-specific binding (substrates phosphorylated by the kinases ataxia telangiectasia-mutated (ATM), ataxia telangiectasia and RAD3-related (ATR) in response to gamma irradiation). In contrast, in vivo two pairs of BRCT domains (3-6) bind to phosphorylated TP53BP1 much more efficiently.
Cellular localizationNucleus matrix. Localizes to DNA damage foci upon ionizing radiation.
- Information by UniProt
- CAGF 28 antibody
- CAGF 29 antibody
- CAGF28 antibody
Lanes 1 & 4 & 7 : Anti-PTIP antibody (ab70434) at 0.25 µg/ml
Lanes 2 & 5 & 8 : Anti-PTIP antibody (ab70434) at 0.1 µg/ml
Lanes 3 & 6 & 9 : Anti-PTIP antibody (ab70434) at 0.025 µg/ml
Lanes 1 & 4 & 7 : Whole cell lysate from 293T cells at 5 µg
Lanes 2 & 5 & 8 : Whole cell lysate from 293T cells at 15 µg
Lanes 3 & 6 & 9 : Whole cell lysate from 293T cells at 50 µg
Developed using the ECL technique.
Predicted band size: 118 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
30ug of whole cell lysate from 293T cells were immunoprecipitated using ab70434 at 10, 5 and 1ug/mg of lysate respectively in lanes 1, 2 and 3. For the subsequent blot ab70434 was used at 0.25ug/ml.
This product has been referenced in:
- Schoonen PM et al. Progression through mitosis promotes PARP inhibitor-induced cytotoxicity in homologous recombination-deficient cancer cells. Nat Commun 8:15981 (2017). Read more (PubMed: 28714471) »
- Xu Y et al. 53BP1 and BRCA1 control pathway choice for stalled replication restart. Elife 6:N/A (2017). Read more (PubMed: 29106372) »