• Product name

  • Description

    Rabbit polyclonal to PTIP
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Pig, Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human PTIP (N terminal). Synthetic peptide mapping to a region between residues 1 and 50 of Human PTIP, using the numbering given in Jowsey, Doherty and Rouse, 2004, J. Biol. Chem. 279(53):55562-55569
    Database link: Q6ZW49-6

  • Positive control

    • Whole cell lysate from 293T cells



Our Abpromise guarantee covers the use of ab70434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2500 - 1/25000. Detects a band of approximately 130 kDa (predicted molecular weight: 118 kDa).
IP Use at 5-10 µg/mg of lysate.


  • Function

    Involved in DNA damage response and in transcriptional regulation through histone methyltransferase (HMT) complexes. Plays a role in early development. In DNA damage response is required for cell survival after ionizing radiation. In vitro shown to be involved in the homologous recombination mechanism for the repair of double-strand breaks (DSBs). Its localization to DNA damage foci requires RNF8 and UBE2N. Recruits TP53BP1 to DNA damage foci and, at least in particular repair processes, effective DNA damage response appears to require the association with TP53BP1 phosphorylated by ATM at 'Ser-25'. Together with TP53BP1 regulates ATM association. Recruits PA1 to sites of DNA damage and the PA1:PAXIP1 complex is required for cell survival in response to DNA damage; the function is probbaly independent of MLL-containing histone methyltransferase (HMT) complexes. Promotes ubiquitination of PCNA following UV irradiation and may regulate recruitment of polymerase eta and RAD51 to chromatin after DNA damage. Proposed to be involved in transcriptional regulation by linking MLL-containing histone methyltransferase (HMT) complexes to gene promoters by interacting with promoter-bound transcription factors such as PAX2. Associates with gene promoters that are known to be regulated by MLL2. During immunoglobulin class switching in activated B cells is involved in trimethylation of histone H3 at 'Lys-4' and in transcription initiation of downstream switch regions at the immunoglobulin heavy-chain (Igh) locus; this function appears to involve the recruitment of MLL-containing HMT complexes.
  • Sequence similarities

    Contains 6 BRCT domains.
  • Domain

    The BRCT 5 and 6 domains function as a single module and are necessary and sufficient for in vitro phospho-specific binding (substrates phosphorylated by the kinases ataxia telangiectasia-mutated (ATM), ataxia telangiectasia and RAD3-related (ATR) in response to gamma irradiation). In contrast, in vivo two pairs of BRCT domains (3-6) bind to phosphorylated TP53BP1 much more efficiently.
  • Cellular localization

    Nucleus matrix. Localizes to DNA damage foci upon ionizing radiation.
  • Information by UniProt
  • Database links

  • Alternative names

    • CAGF 28 antibody
    • CAGF 29 antibody
    • CAGF28 antibody
    • CAGF29 antibody
    • FLJ41049 antibody
    • PACIP 1 antibody
    • PACIP1 antibody
    • PAX interacting (with transcription activation domain) protein 1 antibody
    • PAX interacting protein 1 antibody
    • PAX transactivation activation domain-interacting protein antibody
    • PAX transcription activation domain interacting protein 1 like antibody
    • PAX-interacting protein 1 antibody
    • PAXI1_HUMAN antibody
    • PAXIP 1 antibody
    • PAXIP 1L antibody
    • paxip1 antibody
    • PAXIP1 protein antibody
    • PAXIP1L antibody
    • Protein encoded by CAG trinucleotide repeats antibody
    • PTIP antibody
    • TNRC 2 antibody
    • TNRC2 antibody
    see all


  • Lanes 1 & 4 & 7 : Anti-PTIP antibody (ab70434) at 0.25 µg/ml
    Lanes 2 & 5 & 8 : Anti-PTIP antibody (ab70434) at 0.1 µg/ml
    Lanes 3 & 6 & 9 : Anti-PTIP antibody (ab70434) at 0.025 µg/ml

    Lanes 1 & 4 & 7 : Whole cell lysate from 293T cells at 5 µg
    Lanes 2 & 5 & 8 : Whole cell lysate from 293T cells at 15 µg
    Lanes 3 & 6 & 9 : Whole cell lysate from 293T cells at 50 µg

    Developed using the ECL technique.

    Predicted band size: 118 kDa
    Observed band size: 130 kDa
    why is the actual band size different from the predicted?

    Exposure time: 30 seconds
  • 30ug of whole cell lysate from 293T cells were immunoprecipitated using ab70434 at 10, 5 and 1ug/mg of lysate respectively in lanes 1, 2 and 3. For the subsequent blot ab70434 was used at 0.25ug/ml.


This product has been referenced in:

  • Schoonen PM  et al. Progression through mitosis promotes PARP inhibitor-induced cytotoxicity in homologous recombination-deficient cancer cells. Nat Commun 8:15981 (2017). Read more (PubMed: 28714471) »
  • Xu Y  et al. 53BP1 and BRCA1 control pathway choice for stalled replication restart. Elife 6:N/A (2017). Read more (PubMed: 29106372) »
See all 3 Publications for this product

Customer reviews and Q&As


TheSwissProt database entry for PTIP notes there are 5 isoforms of this protein, one of which is about 90kDa. Although its not clear what the smaller molecular weight bands are.

If you would like I am happy to send you a free replacement of any primary antibody, credit or refundas this vial of ab2614 appears to be recognizing off-target proteins.

Read More

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