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  1. Link

    ptip-overexpression-293t-lysate-whole-cell-ab94247.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair DNA Damage Response BRCT Domain Proteins
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PTIP overexpression 293T lysate (whole cell) (ab94247)

  • Datasheet
  • SDS
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SDS-PAGE - PTIP overexpression 293T lysate (whole cell) (ab94247)
  • Western blot - PTIP overexpression 293T lysate (whole cell) (ab94247)

Overview

  • Product name

    PTIP overexpression 293T lysate (whole cell)
  • General notes

    ab94247 is a 293T cell transfected lysate in which Human PTIP has been transiently over-expressed using a pCMV-PTIP plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes

  • Tested applications

    Suitable for: WBmore details

Properties

  • Mycoplasma free

    Yes
  • Form

    Liquid
  • Storage instructions

    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer

    Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • BRCT Domain Proteins
  • Background

    Function: Involved in DNA damage response and in transcriptional regulation through histone methyltransferase (HMT) complexes. Plays a role in early development. In DNA damage response is required for cell survival after ionizing radiation. In vitro shown to be involved in the homologous recombination mechanism for the repair of double-strand breaks (DSBs). Its localization to DNA damage foci requires RNF8 and UBE2N. Recruits TP53BP1 to DNA damage foci and, at least in particular repair processes, effective DNA damage response appears to require the association with TP53BP1 phosphorylated by ATM at 'Ser-25'. Together with TP53BP1 regulates ATM association. Recruits PA1 to sites of DNA damage and the PA1:PAXIP1 complex is required for cell survival in response to DNA damage; the function is probbaly independent of MLL-containing histone methyltransferase (HMT) complexes. Promotes ubiquitination of PCNA following UV irradiation and may regulate recruitment of polymerase eta and RAD51 to chromatin after DNA damage. Proposed to be involved in transcriptional regulation by linking MLL-containing histone methyltransferase (HMT) complexes to gene promoters by interacting with promoter-bound transcription factors such as PAX2. Associates with gene promoters that are known to be regulated by MLL2. During immunoglobulin class switching in activated B cells is involved in trimethylation of histone H3 at 'Lys-4' and in transcription initiation of downstream switch regions at the immunoglobulin heavy-chain (Igh) locus; this function appears to involve the recruitment of MLL-containing HMT complexes. Similarity: Contains 6 BRCT domains. Domain: The BRCT 5 and 6 domains function as a single module and are necessary and sufficient for in vitro phospho-specific binding (substrates phosphorylated by the kinases ataxia telangiectasia-mutated (ATM), ataxia telangiectasia and RAD3-related (ATR) in response to gamma irradiation). In contrast, in vivo two pairs of BRCT domains (3-6) bind to phosphorylated TP53BP1 much more efficiently.

Applications

Our Abpromise guarantee covers the use of ab94247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Images

  • SDS-PAGE - PTIP overexpression 293T lysate (whole cell) (ab94247)
    SDS-PAGE - PTIP overexpression 293T lysate (whole cell) (ab94247)
    ab94247 at 15µg/lane on an SDS-PAGE gel.
  • Western blot - PTIP overexpression 293T lysate (whole cell) (ab94247)
    Western blot - PTIP overexpression 293T lysate (whole cell) (ab94247)
    All lanes : Anti-PTIP antibody (ab56934) at 1/500 dilution

    Lane 1 : PTIP overexpression 293T lysate (whole cell) (ab94247)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

Protocols

  • Transfected Lysate Preparation Notes

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab94247? Please let us know so that we can cite the reference in this datasheet.

    ab94247 has not yet been referenced specifically in any publications.

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