Key features and details
- Rabbit polyclonal to PTN - C-terminal
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PTN antibody - C-terminal
See all PTN primary antibodies
DescriptionRabbit polyclonal to PTN - C-terminal
Tested applicationsSuitable for: IHC-P, WB, Flow Cytmore details
Species reactivityReacts with: Human
Synthetic peptide within Human PTN aa 125-154 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P21246
- Purchase matching WB positive control:Recombinant Human PTN protein
- NCI-H460 and U251 cell lysates; Human brain tissue; U251 cells.
Previously labelled as Pleiotrophin.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab170606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/500. Predicted molecular weight: 19 kDa.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionHeparin binding mitogenic protein. Has neurite extension activity.
Tissue specificityOsteoblast and brain.
Sequence similaritiesBelongs to the pleiotrophin family.
- Information by UniProt
- HARP antibody
- HB-GAM antibody
- HBBM antibody
Immunohistochemial analysis of formalin-fixed, paraffin-embedded Human brain tissue labeling PTN with ab170606 at 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of U251 cells labeling PTN with ab170606 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
All lanes : Anti-PTN antibody - C-terminal (ab170606) at 1/100 dilution
Lane 1 : U251 cell lysate
Lane 2 : NCI-H460 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 19 kDa
ab170606 has not yet been referenced specifically in any publications.