Recombinant Anti-PTPRE antibody [EPR6715] (ab126788)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6715] to PTPRE
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-PTPRE antibody [EPR6715]
See all PTPRE primary antibodies -
Description
Rabbit monoclonal [EPR6715] to PTPRE -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HL60 treated with TPA, HL60, HeLa and C2C12 cell lysates; Permeabilized HL60 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 5.10 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6715 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126788 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Detects a band of approximately 72 kDa (predicted molecular weight: 71, 75, 81 kDa).
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 72 kDa (predicted molecular weight: 71, 75, 81 kDa). |
Target
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Function
Isoform 1 plays a critical role in signaling transduction pathways and phosphoprotein network topology in red blood cells. May play a role in osteoclast formation and function.
Isoform 2 acts as a negative regulator of insulin receptor (IR) signaling in skeletal muscle. Regulates insulin-induced tyrosine phosphorylation of insulin receptor (IR) and insulin receptor substrate 1 (IRS-1), phosphorylation of protein kinase B and glycogen synthase kinase-3 and insulin induced stimulation of glucose uptake.
Isoform 1 and isoform 2 act as a negative regulator of FceRI-mediated signal transduction leading to cytokine production and degranulation, most likely by acting at the level of SYK to affect downstream events such as phosphorylation of SLP76 and LAT and mobilization of Ca(2+). -
Tissue specificity
Expressed in giant cell tumor (osteoclastoma rich in multinucleated osteoclastic cells). -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Receptor class 4 subfamily.
Contains 2 tyrosine-protein phosphatase domains. -
Domain
The tyrosine-protein phosphatase 2 domain (D2) mediates dimerization. The extreme N- and C- termini of the D2 domain act to inhibit dimerization and removal of these sequences increases dimerization and inhibits enzyme activity. -
Post-translational
modificationsA catalytically active cytoplasmic form (p65) is produced by proteolytic cleavage of either isoform 1, isoform 2 or isoform 3.
Isoform 1 and isoform 2 are phosphorylated on tyrosine residues by tyrosine kinase Neu.
Isoform 1 is glycosylated. -
Cellular localization
Cytoplasm; Cell membrane and Cytoplasm. Predominantly cytoplasmic. A small fraction is also associated with nucleus and membrane. Insulin induces translocation to the membrane. - Information by UniProt
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Database links
- Entrez Gene: 5791 Human
- Entrez Gene: 19267 Mouse
- Entrez Gene: 114767 Rat
- Omim: 600926 Human
- SwissProt: P23469 Human
- SwissProt: P49446 Mouse
- SwissProt: B2GV87 Rat
- Unigene: 127022 Human
see all -
Alternative names
- DKFZp313F1310 antibody
- FLJ57799 antibody
- FLJ58245 antibody
see all
Images
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All lanes : Anti-PTPRE antibody [EPR6715] (ab126788) at 1/1000 dilution
Lane 1 : HL60 cell lysate, treated with TPA
Lane 2 : HL60 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : C2C12 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 71, 75, 81 kDa -
ab126788 at 1/10 dilution staining PTPRE in permeabilized HL60 cells by intracellular flow cytometry (red) compared with a rabbit IgG negative control (green).
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab126788 has been referenced in 1 publication.
- Decotret LR et al. Receptor-type protein tyrosine phosphatase alpha (PTPa) mediates MMP14 localization and facilitates triple-negative breast cancer cell invasion. Mol Biol Cell 32:567-578 (2021). PubMed: 33566639