Product nameAnti-PTP kappa antibody
See all PTP kappa primary antibodies
DescriptionRabbit polyclonal to PTP kappa
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Recombinant fragment
Predicted to work with: Mouse, Human
Synthetic peptide derived from residues 900 - 1000 of Human PTP kappa.
- This antibody gave a positive result in PTP beta (PTPRB) and PTP kappa (PTPRK) recombinant protein domains
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab13225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 50-70 kDa).|
FunctionRegulation of processes involving cell contact and adhesion such as growth control, tumor invasion, and metastasis. Forms complexes with beta-catenin and gamma-catenin/plakoglobin. Beta-catenin may be a substrate for the catalytic activity of PTP-kappa.
Tissue specificityHigh levels in lung, brain and colon; less in liver, pancreas, stomach, kidney, placenta and mammary carcinoma.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Receptor class 2B subfamily.
Contains 4 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 MAM domain.
Contains 2 tyrosine-protein phosphatase domains.
modificationsThis protein undergoes proteolytic processing.
Cellular localizationCell junction > adherens junction. Cell membrane.
- Information by UniProt
- dJ480J14.2.1 antibody
- DKFZp686C2268 antibody
- DKFZp779N1045 antibody
All lanes : Anti-PTP kappa antibody (ab13225) at 1 µg/ml
Lane 1 : 0.1µg GLEPP1 (PTPRO) protein
Lane 2 : 0.1µg SAP 1 (PTPRH) protein
Lane 3 : 0.1µg PTP gamma (PTPRG) protein
Lane 4 : 0.1µg PTP beta (PTPRB) protein
Lane 5 : 0.1µg DEP1 (PTPRJ) protein
Lane 6 : 0.1µg PTP kappa (PTPRK) protein
Lane 7 : 0.1µg PCPTP1 (PTPRR) protein
Lane 8 : 0.1µg LAR (PTPRF) protein
Performed under reducing conditions.
Predicted band size: 50-70 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Recombinant PTP phosphatase domains (0.1
g of protein) were probed with ab13225 at 1/1000. The antibody recognises the recombinant PTP kappa fusion protein at ~65 kDa. Ladder sizes are in kilodaltons. µ
Lane # Recombinant Protein
1 GLEPP1 (PTP RO)
2 SAP 1 (PTP RH)
3 PTP gamma (PTP RG)
4 PTP beta (PTP B)
This product has been referenced in:
- Agarwal S et al. Tumor derived mutations of protein tyrosine phosphatase receptor type k affect its function and alter sensitivity to chemotherapeutics in glioma. PLoS One 8:e62852 (2013). WB ; Human . Read more (PubMed: 23696788) »
- Flavell JR et al. Down-regulation of the TGF-beta target gene, PTPRK, by the Epstein-Barr virus encoded EBNA1 contributes to the growth and survival of Hodgkin lymphoma cells. Blood 111:292-301 (2008). Read more (PubMed: 17720884) »