Key features and details
- Rabbit polyclonal to PTP lambda/FMI
- Suitable for: WB, IHC-P, ICC/IF
- Isotype: IgG
Product nameAnti-PTP lambda/FMI antibody
Predicted to work with: Human
Synthetic peptide corresponding to Human PTP lambda/FMI aa 900-1000 (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- IF: HeLa Hek293, HepG2 and MCF7 cells. IHC-P: human cerebral cortex WB: human brain, kidney,pancreas, heart tissue lysates.
This product was previously labelled as PTP lambda
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
|WB||1/500. Predicted molecular weight: 160 kDa.Can be blocked with Human PTP lambda/FMI peptide (ab13230).|
|IHC-P||1/75. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 5 µg/ml.|
RelevancePTP lambda is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle and oncogenic transformation. PTP lambda is a large protein that possesses a C-terminus PTP domain and multiple noncatalytic domains which include a domain with similarity to band 4.1 superfamily of cytoskeletal-associated proteins, a region consisting of five PDZ domains and a leucine zipper motif. Four alternatively spliced transcript variants, which encode distinct proteins, have been reported. PTP lambda is up-regulated upon cell contact and is found in high levels in brain, pancreas, and skeletal muscle; less in colon, kidney, liver, stomach and uterus. It is not expressed in placenta and spleen.
Cellular localizationType 1 membrane protein
- Pancreatic carcinoma phosphatase 2 antibody
- PCP-2 antibody
- Protein-tyrosine phosphatase J antibody
A weak band of predicted size (~160kDa) is observed in human kidney lysate which is quenched by the blocking peptide (ab13230).
Image courtesy of www.proteinatlas.org
ab13223 staining PTP lambda/FMI in human cerebral cortex and showing a strong staining on the neuronal cells. Paraffin embedded human cerebral cortex tissues were cut into 4µm sections and incubated with ab13223 (1/75) for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab13223 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
ICC/IF image of ab13223 stained HeLa cells. The cells were 100% methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab13223, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in 4% PFA and 100% methanol fixed Hek293, HepG2 and MCF7 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab13223 has been referenced in 2 publications.
- Zhu Z et al. Protein tyrosine phosphatase receptor U (PTPRU) is required for glioma growth and motility. Carcinogenesis 35:1901-10 (2014). PubMed: 24876153
- Hyun SW et al. Diverse injurious stimuli reduce protein tyrosine phosphatase-µ expression and enhance epidermal growth factor receptor signaling in human airway epithelia. Exp Lung Res 37:327-43 (2011). PubMed: 21649524