Overview

  • Product name
  • Description
    Goat polyclonal to PTP rho
  • Host species
    Goat
  • Specificity
    This antibody is expected to recognise both reported isoforms.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    (C)NTWEKPMLDQAV

    , corresponding to amino acids 58 - 69 of Human PTP rho.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab23936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/16000.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 150 kDa.

Target

  • Function
    May be involved in both signal transduction and cellular adhesion in the CNS.
  • Tissue specificity
    Expressed in colon, lung, heart and testis, as well as in fetal and adult brain. Not detected in muscle and peripheral blood leukocytes.
  • Sequence similarities
    Belongs to the protein-tyrosine phosphatase family. Receptor class 2B subfamily.
    Contains 4 fibronectin type-III domains.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 MAM domain.
    Contains 2 tyrosine-protein phosphatase domains.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Protein tyrosine phosphatase receptor type T antibody
    • PTPRT antibody
    • PTPRT_HUMAN antibody
    • R PTP T antibody
    • R-PTP-T antibody
    • Receptor protein tyrosine phosphatase antibody
    • Receptor-type tyrosine-protein phosphatase rho antibody
    • Receptor-type tyrosine-protein phosphatase T antibody
    • RPTP-rho antibody
    • RPTPmam4 antibody
    • RPTPrho antibody
    see all

Images

  • Anti-PTP rho antibody (ab23936) at 1.5 µg/ml
    Performed under reducing conditions.


    ab23936 staining Human Brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

References

ab23936 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (colon cancer cell-line)
Specification
colon cancer cell-line
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2%

Abcam user community

Verified customer

Submitted Jan 20 2006

Answer

Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to go ahead and purchase ab23936, please let us know how you get on by submitting an Abreview and in return we will offer you 50 Abpoints which can be redeemed on a number of rewards (a further 100 Abpoints will be offered for an image). For ab13228, I strongly urge whoever used the antibody to submit feedback to us. Because it is a fast-track antibody, we will award 1,000 Abpoints to the first researcher who sends us positive feedback (including an image and details of materials & methods). Conclusive negative feedback that leads us to withdraw the antibody will also be rewarded with 1,000 Abpoints. Other useful positive and negative feedback will be rewarded with 50 Abpoints. All awards are subject to approval by Abcam scientists and provision of suitable data. Please submit feedback to fast-track@abcam.com

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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