Overview

  • Product name

    Anti-PTP zeta/ Phosphacan antibody
    See all PTP zeta/ Phosphacan primary antibodies
  • Description

    Rabbit polyclonal to PTP zeta/ Phosphacan
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence: LFRHLHTVSQ ILPQVTSATE SDKVPLHASL PVAGGDLLLE PSLAQYSDVL STTHAASETL EFGSESGVLY KTLMFSQVEP PSSDAMMHAR SSGPEPSYAL SDNEGSQHIF TVSYSSAIPV HDSVGVTYQG SLFSGPSHIP IPKSSLIT corresponding to internal amino acids 831-978 of Human PTP zeta/ Phosphacan.

  • Positive control

    • Human cerebral cortex tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab126497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 255 kDa.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    May be involved in the regulation of specific developmental processes in the CNS.
  • Tissue specificity

    Specifically expressed in the central nervous system, where it is localized in the Purkinje cell layer of the cerebellum, the dentate gyrus, and the subependymal layer of the anterior horn of the lateral ventricle. Developmentally regulated in the brain.
  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family. Receptor class 5 subfamily.
    Contains 1 alpha-carbonic anhydrase domain.
    Contains 1 fibronectin type-III domain.
    Contains 2 tyrosine-protein phosphatase domains.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3F8 chondroitin sulfate proteoglycan antibody
    • 3H1 keratan sulfate proteoglycan antibody
    • HPTPZ antibody
    • HPTPzeta antibody
    • Phosphacan antibody
    • Protein tyrosine phosphatase receptor type Z polypeptide 2 antibody
    • Protein tyrosine phosphatase, receptor type, Z polypeptide 1 antibody
    • Protein tyrosine phosphatase, receptor type, zeta polypeptide 1 antibody
    • Protein-tyrosine phosphatase receptor type Z polypeptide 1 antibody
    • Protein-tyrosine phosphatase receptor type Z polypeptide 2 antibody
    • PTP-ZETA antibody
    • PTP18 antibody
    • PTPRZ antibody
    • PTPRZ_HUMAN antibody
    • Ptprz1 antibody
    • PTPZ antibody
    • R PTP zeta 2 antibody
    • R-PTP-zeta antibody
    • R-PTP-zeta-2 antibody
    • Receptor type tyrosine phosphatase beta/zeta antibody
    • Receptor-type tyrosine-protein phosphatase zeta antibody
    • RPTP-BETA antibody
    • RPTPB antibody
    • RPTPbeta antibody
    see all

Images

  • ab126497 at 1/50 dilution staining PTP zeta/ Phosphacan in paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.
  • Anti-PTP zeta/ Phosphacan antibody (ab126497) + Human brain tissue

    Predicted band size: 255 kDa

  • Immunofluorescent staining of Human cell line A-431 shows positivity in vesicles. Recommended concentration of ab126497 1-4 µg/ml. Cells treated with PFA/Triton X-100.

References

This product has been referenced in:

  • Huang P  et al. Chemotherapy-driven increases in the CDKN1A/PTN/PTPRZ1 axis promote chemoresistance by activating the NF-?B pathway in breast cancer cells. Cell Commun Signal 16:92 (2018). Read more (PubMed: 30497491) »
  • Yuzwa SA  et al. Developmental Emergence of Adult Neural Stem Cells as Revealed by Single-Cell Transcriptional Profiling. Cell Rep 21:3970-3986 (2017). Read more (PubMed: 29281841) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Brain - Amygdala and ECx)
Specification
Brain - Amygdala and ECx
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton-X(0.5%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 01 2012

Question
Answer

I am very pleased to hear you would like to accept our offer and test ab126497 inIHC-FrFl. This code will give you 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abreview for [IHC-FrFl and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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