Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1836(2)] to PTP1B - BSA and Azide free
- Knockout validated
- Reacts with: Human
Product nameAnti-PTP1B antibody [EPR1836(2)] - BSA and Azide free
See all PTP1B primary antibodies
DescriptionRabbit monoclonal [EPR1836(2)] to PTP1B - BSA and Azide free
Species reactivityReacts with: Human
Synthetic peptide within Human PTP1B aa 350-450. The exact sequence is proprietary.
Database link: P18031
- WB: Wild-type HAP1 cell lysate; Jurkat and MCF7 cell lysate.
ab232050 is the carrier-free version of ab133244 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab232050 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
FunctionMay play an important role in CKII- and p60c-src-induced signal transduction cascades.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
Contains 1 tyrosine-protein phosphatase domain.
modificationsOxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- PTP1B antibody
- Non receptor tyrosine phosphatase 1 antibody
- Protein phosphotyrosylphosphatase 1B antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PTP1B knockout HAP1 cell lysate (20 µg)
Lane 3: Jurkat cell lysate (20 µg)
Lane 4: MCF7 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab133244 observed at 54 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab133244 was shown to recognize PTP1B when PTP1B knockout samples were used, along with additional cross-reactive bands. Wild-type and PTP1B knockout samples were subjected to SDS-PAGE. ab133244 and ab8245 (loading control to GAPDH) were diluted 1/1,000 and 1/2,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133244).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232050 has not yet been referenced specifically in any publications.