Overview

  • Product name

    Anti-PTP1B antibody [OTI1D10]
    See all PTP1B primary antibodies
  • Description

    Mouse monoclonal [OTI1D10] to PTP1B
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Dog, Human, Monkey
  • Immunogen

    Recombinant full length protein corresponding to Human PTP1B.
    Database link: NP_002818

  • Positive control

    • WB: PTP1B-transfected HEK293T, HepG2, HeLa, COS7, Jurkat, MDCK, PC12, and MCF7 cell lysates IHC-P: Human colon, Human lymph node and Human lymphoma tissues ICC/IF: COS7 cells transiently transfected by PTP1B FC: PTP1B-transfected HEK293T cells
  • General notes

    The clone number has been updated from 1D10 to OTI1D10, both clone numbers name the same clone.

Properties

Applications

Our Abpromise guarantee covers the use of ab124375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 50 kDa.
IHC-P 1/150.
Flow Cyt 1/100.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100.

Target

  • Function

    May play an important role in CKII- and p60c-src-induced signal transduction cascades.
  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
    Contains 1 tyrosine-protein phosphatase domain.
  • Post-translational
    modifications

    Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • PTP1B antibody
    • Non receptor tyrosine phosphatase 1 antibody
    • Protein phosphotyrosylphosphatase 1B antibody
    • Protein tyrosine phosphatase 1B antibody
    • Protein tyrosine phosphatase non receptor type 1 antibody
    • Protein tyrosine phosphatase placental antibody
    • Protein-tyrosine phosphatase 1B antibody
    • PTN1_HUMAN antibody
    • PTP 1B antibody
    • PTP-1B antibody
    • PTPN 1 antibody
    • PTPN1 antibody
    • Tyrosine protein phosphatase non receptor type 1 antibody
    • Tyrosine-protein phosphatase non-receptor type 1 antibody
    see all

Images

  • All lanes : Anti-PTP1B antibody [OTI1D10] (ab124375)

    Lane 1 : Wild-type HeLa cells
    Lane 2 : PTPB1 knockout HeLa cells

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 50 kDa

  • Staining of PTP1B in Paraffin-embedded Human colon tissue by Immunohistochemistry using ab124375 at a dilution of 1/150.
  • All lanes : Anti-PTP1B antibody [OTI1D10] (ab124375) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY PTP1B

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 50 kDa

  • All lanes : Anti-PTP1B antibody [OTI1D10] (ab124375) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : SVT2 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 50 kDa

  • Staining of PTP1B in Paraffin-embedded Human lymph node tissue by Immunohistochemistry using ab124375 at a dilution of 1/150.
  • Staining of PTP1B in Paraffin-embedded Human lymphoma tissue by Immunohistochemistry using ab124375 at a dilution of 1/150.
  • Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTP1B using ab124375 at a dilution of 1/100.
  • HEK293T cells transfected with either PTP1B overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab124375 at a dilution of 1/100, and then analyzed by flow cytometry.

References

This product has been referenced in:

  • Gabay O  et al. Sirtuin 1 enzymatic activity is required for cartilage homeostasis in vivo in a mouse model. Arthritis Rheum 65:159-66 (2013). IHC ; Mouse . Read more (PubMed: 23124828) »
See 1 Publication for this product

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