Product nameAnti-PTP1B (phospho S378) antibody [EP1840Y]
See all PTP1B primary antibodies
DescriptionRabbit monoclonal [EP1840Y] to PTP1B (phospho S378)
Specificityab76239 detects PTP1B phosphorylated on serine 378.
Tested applicationsSuitable for: WB, IHC-P, ICCmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
A phospho-specific peptide corresponding to residues surrounding serine 378 of human PTP1B
- HeLa cytoplasmic or nuclear lysate treated with calyculin A Human breast carcinoma tissue
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab76239 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary.
WB: 1/500000 - 1/1000000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
Is unsuitable for Flow Cyt or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay play an important role in CKII- and p60c-src-induced signal transduction cascades.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
Contains 1 tyrosine-protein phosphatase domain.
modificationsOxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- PTP1B antibody
- Non receptor tyrosine phosphatase 1 antibody
- Protein phosphotyrosylphosphatase 1B antibody
All lanes : Anti-PTP1B (phospho S378) antibody [EP1840Y] (ab76239) at 1/1000000 dilution
Lane 1 : HeLa cell lysates - untreated
Lane 2 : HeLa cell lysates - treated with calyculin A
Lysates/proteins at 10 µg per lane.
All lanes : goat anti-rabbit HRP at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
ab76239, at a 1/50 dilution, staining human PTP1B (phospho S378) in breast carcinoma by Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.