Overview

  • Product name

    Anti-PTP4A3/PRL-R antibody
    See all PTP4A3/PRL-R primary antibodies
  • Description

    Rabbit polyclonal to PTP4A3/PRL-R
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human PTP4A3/PRL-R aa 160-169 conjugated to keyhole limpet haemocyanin (Cysteine residue).
    Sequence:

    FKDPHTHKTR

  • Positive control

    • Whole extract of human HepG2 cells, protease digested formalin-fixed, paraffin-embedded human heart sections and recombinant human PRL-3.
  • General notes

    Previously labelled as PTP4A3. 

Properties

Applications

Our Abpromise guarantee covers the use of ab50276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 19 kDa. Staining can be inhibited by the immunizing peptide.
IHC-P 1/250 - 1/500.
IP Use at an assay dependent concentration.

0.5 - 1.0 μL/test

ICC/IF Use at an assay dependent concentration. PubMed: 22345654

Target

  • Function

    Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. Enhances cell proliferation, cell motility and invasive activity, and promotes cancer metastasis. May be involved in the progression of cardiac hypertrophy by inhibiting intracellular calcium mobilization in response to angiotensin II.
  • Tissue specificity

    Mainly expressed in cardiomyocytes and skeletal muscle; also found in pancreas. Consistently overexpressed in colon cancer metastasis.
  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family.
    Contains 1 tyrosine-protein phosphatase domain.
  • Post-translational
    modifications

    Farnesylated. Farnesylation is required for membrane targeting.
  • Cellular localization

    Cell membrane. Early endosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • Potentially prenylated protein tyrosine phosphatase antibody
    • PRL 3 antibody
    • PRL R antibody
    • PRL-3 antibody
    • PRL-R antibody
    • PRL3 antibody
    • PRLR antibody
    • Protein tyrosine phosphatase 4a3 antibody
    • Protein tyrosine phosphatase type IVA 3 antibody
    • Protein-tyrosine phosphatase 4a3 antibody
    • Protein-tyrosine phosphatase of regenerating liver 3 antibody
    • PTP 4A3 antibody
    • PTP4A3 antibody
    • TP4A3_HUMAN antibody
    see all

References

This product has been referenced in:

  • Edwards DR  et al. PRL-3 increases the aggressive phenotype of prostate cancer cells in vitro and its expression correlates with high-grade prostate tumors in patients. Int J Oncol 52:402-412 (2018). Read more (PubMed: 29207031) »
  • Zhang Y  et al. PRL-3 promotes gastric cancer peritoneal metastasis via the PI3K/AKT signaling pathway in vitro and in vivo. Oncol Lett 15:9069-9074 (2018). Read more (PubMed: 29805638) »
See all 11 Publications for this product

Customer reviews and Q&As

Question

LOT NUMBER -- NOT SPECIFIED -- ORDER NUMBER 932341 DESCRIPTION OF THE PROBLEM High background SAMPLE HCT116, Cell extract PRIMARY ANTIBODY Concentration or dilution 1:500 Diluent buffer 1% BSA in PBS Incubation time overnight Incubation temperature 4 º C Washing: Buffer Used Tris buffered saline Number of washes 3 washes , 15 min. each DETECTION METHOD Pierce pico supersignal POSITIVE AND NEGATIVE CONTROLS USED Positive control Beta actin ANTIBODY STORAGE CONDITIONS 4 degC SAMPLE PREPARATION Lysis buffer Laemelli’s buffer Protease inhibitors no Phosphatase inhibitors no Reducing agent SDS , Beta -mercaptoethanol Boiling for ≥5 min? 5 min. ELECTROPHORESIS/GEL CONDITIONS Reducing or Non Reducing gel Reducing Percentage of gel 12% Volts applied 60 V Time applied Overnight TRANSFER AND BLOCKING CONDITIONS Type of membrane nitrocellulose Protein transfer verified yes Blocking agent and concentration 5 % milk Blocking time 1 hr. Blocking temperature 37 º C SECONDARY ANTIBODY Species rabbit Reacts against Mouse , human Concentration or dilution 1:5000 Diluent buffer 2.5% milk Incubation time 1 hr. Incubation temperature: 37 º C Fluorochrome or enzyme conjugate HRP Washing: Buffer Used TBS Number of washes 3 washes, 5 min. each HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 7 DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? We have tried blocking with BSA instead , also tried to increase dilution of primary and secondary antibody , but it did not work.

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Answer

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality. Reviewing this case, I would like to offer some suggestions to help optimise the results from ab50276. I would also appreciate if you can confirm some further details: 1. I can suggest it is important to aliquot and store the antibody as soon as possible at -20oC as recommended on the datasheet. This will ensure the antibody remains stable and also that we can continue to provide our Abpromise guarantee. 2. I can suggest to try a lysis buffer such as RIPA. This may provide a more suitable preparation in this case for PTP4A3. It is rare to use Laemmli buffer alone for lysing, and this may not be enough. 3. We recommend not to mix blocking agents in an experiment. Using one on its own can often improve the results. I can suggest to try BSA. 4. I can suggest to try including 0.2% Tween 20 in the buffers. This will help to solubilise the antibody and also to wash away any excess antibody. 5. The protein size, 19 kDa, is quite small. Could you confirm if a molecular weight marker been used to ensure this has not run off the gel? In the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund. I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

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