Product nameAnti-PTPD1 antibody
See all PTPD1 primary antibodies
DescriptionRabbit polyclonal to PTPD1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
- This antibody gave a positive result in IHC in the following FFPE tissue: Human normal testis.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab12550 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa).|
|IHC-P||1/600. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
RelevancePTPD1 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. The predicted PTPD1 protein has 1,174 amino acids. Its overall structure is similar to the structures of PTPH1 and PTPMEG1 (PTPN4), with an N-terminal ezrin-like domain and a single PTP domain at the extreme C terminus. Northern blot analysis shows that PTPD1 is expressed abundantly in human placenta, lung, and skeletal muscle, and to a lesser extent in several other tissues. PTPD1 may have a role in liver regeneration and spermatogenesis.
Cellular localizationCytoplasmic, Cytoskeleton
- Protein tyrosine phosphatase D1 antibody
- Protein tyrosine phosphatase non receptor type 21 antibody
- PTPN 21 antibody
All lanes : Anti-PTPD1 antibody (ab12550) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab27251)
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 133 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa, 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
Image courtesy of Human Protein Atlas
ab12550 staining PTPD1 in Human kidney. Paraffin embedded human skin tissue was incubated with ab12550 (1/600 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Ab12550 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.
Further results for this antibody can be found at www.proteinatlas.org
ab12550 has not yet been referenced specifically in any publications.