Key features and details
- Rabbit polyclonal to PTPD1/PTPN21
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PTPD1/PTPN21 antibody
DescriptionRabbit polyclonal to PTPD1/PTPN21
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
- This antibody gave a positive result in IHC in the following FFPE tissue: Human normal testis.
This product was previously labelled as PTPD1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab12550 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa).|
RelevancePTPD1 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. The predicted PTPD1 protein has 1,174 amino acids. Its overall structure is similar to the structures of PTPH1 and PTPMEG1 (PTPN4), with an N-terminal ezrin-like domain and a single PTP domain at the extreme C terminus. Northern blot analysis shows that PTPD1 is expressed abundantly in human placenta, lung, and skeletal muscle, and to a lesser extent in several other tissues. PTPD1 may have a role in liver regeneration and spermatogenesis.
Cellular localizationCytoplasmic, Cytoskeleton
- Protein tyrosine phosphatase D1 antibody
- Protein tyrosine phosphatase non receptor type 21 antibody
- PTPN 21 antibody
All lanes : Anti-PTPD1/PTPN21 antibody (ab12550) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab27251)
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 133 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa, 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
ab12550 has not yet been referenced specifically in any publications.