Key features and details
- Rabbit polyclonal to PTPIP51
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PTPIP51 antibody
See all PTPIP51 primary antibodies
DescriptionRabbit polyclonal to PTPIP51
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
- WB: Caco-2 cell lysate. ICC/IF: U-2 OS cells. IHC-P: Human testis and skin tissue.
This product was previously labelled as FAM82A2
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224081 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 52 kDa.|
|IHC-P||1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceFAM82A2, or family with sequence similarity 82, member C, may participate in differentiation and apoptosis of keratinocytes. Overexpression of FAM82A2 induces apoptosis.
Cellular localizationMitochondrion membrane; Single-pass membrane protein. Cytoplasm. Nucleus. Note=In interphase localizes in the cytoplasm, and during mitosis localizes to the spindle microtubules and spindle poles.
- Cerebral protein 10 antibody
- FAM82C antibody
- FAM82C, formerly antibody
Anti-PTPIP51 antibody (ab224081) at 1/250 dilution + Caco-2 (human colorectal adenocarcinoma cell line) cell lysate
Predicted band size: 52 kDa
Paraffin-embedded human testis tissue staining PTPIP51 with ab224081 at 1/500 dilution in immunohistochemical analysis. Strong cytoplasmic positibity in seminiferous duct cells.
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for PTPIP51 (green) using ab224081 (4 µg/ml) in ICC/IF.
Paraffin-embedded human skeletal muscle tissue staining PTPIP51 with ab224081 at 1/500 dilution in immunohistochemical analysis. Weak cytoplasmic positivity in myocytes.
Paraffin-embedded human skin tissue staining PTPIP51 with ab224081 at 1/500 dilution in immunohistochemical analysis. Moderate to strong cytoplasmic positibity in keratinocytes.
Paraffin embedded human testis tissue stained for PTPIP51 with ab224081 (1/1000 dilution) in immunohistochemical analysis.
ab224081 has not yet been referenced specifically in any publications.