Key features and details
- Rabbit polyclonal to PTPLB
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PTPLB antibody
DescriptionRabbit polyclonal to PTPLB
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
- WB: MCF7 and LO2 whole cell lysate. IHC-P: Human prostate cancer tissue. ICC/IF: MCF7 cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab237046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/2000.|
|ICC/IF||1/50 - 1/200.|
FunctionResponsible for the dehydration step in very long-chain fatty acids (VLCFAs) synthesis.
Tissue specificityHighly expressed in testis, spleen, prostate, colon and heart, followed by moderate expression in thymus, ovary, small intestine, peripheral blood leukocytes, liver, skeletal muscle and pancreas. Weakly detected in kidney, placenta, brain and lung.
Sequence similaritiesBelongs to the very long-chain fatty acids dehydratase HACD family.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- 3 hydroxyacyl CoA dehydratase 2 antibody
- 3-hydroxyacyl-CoA dehydratase 2 antibody
- HACD2 antibody
All lanes : Anti-PTPLB antibody (ab237046) at 1/500 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : LO2 whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Paraffin-embedded human prostate cancer tissue stained for PTPLB using ab237046 at 1/100 dilution in immunohistochemical analysis.
MCF7 (Human breast adenocarcinoma cell line) cells stained for PTPLB (Green) using ab237046 at 1/100 dilution in ICC/IF, followed by an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237046 has not yet been referenced specifically in any publications.