Key features and details
- Rabbit polyclonal to PTPN12
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PTPN12 antibody
See all PTPN12 primary antibodies
DescriptionRabbit polyclonal to PTPN12
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
- WB: U-251 MG cell lysate. ICC/IF: U-251 MG cells. IHC-P: Human cerebellum tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 73,74,88 kDa.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 4 subfamily.
Contains 1 tyrosine-protein phosphatase domain.
- Information by UniProt
- Protein tyrosine phosphatase G1 antibody
- Protein tyrosine phosphatase non receptor type 12 antibody
- Protein-tyrosine phosphatase G1 antibody
Anti-PTPN12 antibody (ab224068) at 1/100 dilution + U-251 MG (human brain glioma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 73,74,88 kDa
PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for PTPN12 (green) using ab224068 at 4 µg/ml in ICC/IF.
Paraffin-embedded human cerebellum tissue stained for PTPN12 using ab224068 at 1/200 dilution in immunohistochemical analysis.
ab224068 has not yet been referenced specifically in any publications.